Antibodies against dengue virus E protein peptide bind to human plasminogen and inhibit plasmin activity

Y. H. Huang, B. I. Chang, H. Y. Lei, H. S. Liu, C. C. Liu, H. L. Wu, T. M. Yeh

研究成果: Article

35 引文 斯高帕斯(Scopus)

摘要

Both mice and rabbits immunized with dengue virus E protein peptide spanning amino acids 100-119 (D4E) produced antibodies that reacted not only with the D4E peptide itself but also with human plasminogen, as shown by ELISA and Western blot. Sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against D4E and plasminogen. Furthermore, such sera all contained plasmin inhibitory activity. Using affinity-purified anti-D4E antibodies and free D4E peptide for competitive inhibition, we demonstrated that the inhibition of plasmin activity was due to anti-D4E antibodies rather than other substances in the sera. Taken together, these results suggest dengue virus E protein amino acids 100-119 are a cross-reactive immunogenic region, and antibodies against this region may interfere with human fibrinolysis.

原文English
頁(從 - 到)35-40
頁數6
期刊Clinical and Experimental Immunology
110
發行號1
DOIs
出版狀態Published - 1997 一月 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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