摘要
Both mice and rabbits immunized with dengue virus E protein peptide spanning amino acids 100-119 (D4E) produced antibodies that reacted not only with the D4E peptide itself but also with human plasminogen, as shown by ELISA and Western blot. Sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against D4E and plasminogen. Furthermore, such sera all contained plasmin inhibitory activity. Using affinity-purified anti-D4E antibodies and free D4E peptide for competitive inhibition, we demonstrated that the inhibition of plasmin activity was due to anti-D4E antibodies rather than other substances in the sera. Taken together, these results suggest dengue virus E protein amino acids 100-119 are a cross-reactive immunogenic region, and antibodies against this region may interfere with human fibrinolysis.
原文 | English |
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頁(從 - 到) | 35-40 |
頁數 | 6 |
期刊 | Clinical and Experimental Immunology |
卷 | 110 |
發行號 | 1 |
DOIs | |
出版狀態 | Published - 1997 |
All Science Journal Classification (ASJC) codes
- 一般醫學