Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation

Ya Ting Chu, Shu Wen Wan, Yu Chang Chang, Chien Kuo Lee, Betty A. Wu-Hsieh, Robert Anderson, Yee Shin Lin

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)


Dengue virus (DENV) infection causes dengue fever, dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS). DHF/DSS patients have been reported to have increased levels of urinary histamine, chymase, and tryptase, which are major granule-associated mediators from mast cells. Previous studies also showed that DENV-infected human mast cells induce production of proinflammatory cytokines and chemokines, suggesting a role played by mast cells in vascular perturbation as well as leukocyte recruitment. In this study, we show that DENV but not UV-inactivated DENV enhanced degranulation of mast cells and production of chemokines (MCP-1, RANTES, and IP-10) in a mouse model. We have previously shown that antibodies (Abs) against a modified DENV nonstructural protein 1 (NS1), designated DJ NS1, provide protection in mice against DENV challenge. In the present study, we investigate the effects of DJ NS1 Abs on mast cell-associated activities. We showed that administration of anti-DJ NS1 Abs into mice resulted in a reduction of mast cell degranulation and macrophage infiltration at local skin DENV infection sites. The production of DENV-induced chemokines (MCP-1, RANTES, and IP-10) and the percentages of tryptase-positive activated mast cells were also reduced by treatment with anti-DJ NS1 Abs. These results indicate that Abs against NS1 protein provide multiple therapeutic benefits, some of which involve modulating DENV-induced mast cell activation.

頁(從 - 到)602-614
期刊Laboratory Investigation
出版狀態Published - 2017 5月 1

All Science Journal Classification (ASJC) codes

  • 病理學與法醫學
  • 分子生物學
  • 細胞生物學


深入研究「Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation」主題。共同形成了獨特的指紋。