Antioxidant activity of linear and star-shaped polypeptides modified with dopamine and glutathione

Ching Fang Su, Yu Fon Chen, Yi Jen Tsai, Shao Ming Weng, Jeng Shiung Jan

研究成果: Article同行評審

摘要

The aim of this study was to investigate the effect of topology on the antioxidative activities of antioxidant-grafted polypeptides. The linear and star-shaped poly(L-glutamic acid) (PLG) polypeptides were synthesized by N-carboxyanhydrides (NCAs) ring opening polymerization (ROP) using primary amine and polyols as initiators, followed by deprotection. Graft copolypeptides (PLG-Dopa, PLG-GSH and PLG-Dopa-GSH) were obtained by EDC/NHS coupling chemistry using dopamine (Dopa) and glutathione (GSH). Successful synthesis of these polypeptides was confirmed by 1H NMR, MALDI TOF, GPC, UV–vis and IR spectra analyses. The conformation of polypeptides was investigated by circular dichroism (CD) spectroscopy, demonstrating the grafting of Dopa and GSH onto PLG was accompanied by a conformational change from a random coil to α-helical structure. The results showed that the antioxidant activities of PLG-Dopa and PLG-Dopa-GSH were mainly ascribed to Dopa. It was found that the conjugation of Dopa resulted in the decrease in its antioxidant activity and the antioxidant-grafted polypeptides exhibited comparable antioxidant activity regardless the polypeptide arm number. The conjugation of GSH could prolong polypeptide antioxidant activity by preventing Dopa from oxidation. It was found that both PLG-Dopa and PLG-Dopa-GSH exhibited good hemocompatibility and biocompatibility.

原文English
文章編號110497
期刊European Polymer Journal
152
DOIs
出版狀態Published - 2021 六月 5

All Science Journal Classification (ASJC) codes

  • 物理與天文學 (全部)
  • 聚合物和塑料
  • 有機化學
  • 材料化學

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