Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo

Sin Guang Chen, Mei Ling Cheng, Kuan Hsing Chen, Jim Tong Horng, Ching-Chuan Liu, Shih-Min Wang, Hiroaki Sakurai, Yann Lii Leu, Shulhn Der Wang, Hung Yao Ho

研究成果: Article

8 引文 (Scopus)

摘要

No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2A pro ; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.

原文English
文章編號935
期刊Scientific reports
7
發行號1
DOIs
出版狀態Published - 2017 十二月 1

指紋

Lamiaceae
Enterovirus
Antiviral Agents
Heterogeneous-Nuclear Ribonucleoproteins
Reactive Oxygen Species
Phosphotransferases
Enterovirus Infections
Eukaryotic Initiation Factors
In Vitro Techniques
Chinese Traditional Medicine
Viral RNA
Viral Proteins
Cytoplasm
Peptide Hydrolases
Therapeutics
Viruses
Food
Membranes
Health

All Science Journal Classification (ASJC) codes

  • General

引用此文

Chen, Sin Guang ; Cheng, Mei Ling ; Chen, Kuan Hsing ; Horng, Jim Tong ; Liu, Ching-Chuan ; Wang, Shih-Min ; Sakurai, Hiroaki ; Leu, Yann Lii ; Wang, Shulhn Der ; Ho, Hung Yao. / Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. 於: Scientific reports. 2017 ; 卷 7, 編號 1.
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abstract = "No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2A pro ; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.",
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Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. / Chen, Sin Guang; Cheng, Mei Ling; Chen, Kuan Hsing; Horng, Jim Tong; Liu, Ching-Chuan; Wang, Shih-Min; Sakurai, Hiroaki; Leu, Yann Lii; Wang, Shulhn Der; Ho, Hung Yao.

於: Scientific reports, 卷 7, 編號 1, 935, 01.12.2017.

研究成果: Article

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AU - Chen, Sin Guang

AU - Cheng, Mei Ling

AU - Chen, Kuan Hsing

AU - Horng, Jim Tong

AU - Liu, Ching-Chuan

AU - Wang, Shih-Min

AU - Sakurai, Hiroaki

AU - Leu, Yann Lii

AU - Wang, Shulhn Der

AU - Ho, Hung Yao

PY - 2017/12/1

Y1 - 2017/12/1

N2 - No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2A pro ; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.

AB - No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2A pro ; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.

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