Application of Chitinous materialss in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015

Tzu Wen Liang, Shan Ni Jen, Anh Dzung Nguyen, San Lang Wang

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(L-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.

原文English
頁(從 - 到)1-11
頁數11
期刊Polymers
8
發行號3
DOIs
出版狀態Published - 2016 3月 22

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 聚合物和塑料

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