Application of molecular biological tools for monitoring efficiency of trichloroethylene remediation

Yi Ju Wu, Pao Wen Grace Liu, You Siang Hsu, Liang Ming Whang, Tsair Fuh Lin, Wei Nung Hung, Kun Ching Cho

研究成果: Article

1 引文 (Scopus)

摘要

Trichloroethylene (TCE) is one of the most ubiquitous halogenated organic compounds of concerns of carcinogens in groundwater in Taiwan. Bioremediation has been recognized as a cost-effective approach in reducing TCE concentration. Five pilot-scale wells were constructed to monitor TCE concentrations in contaminated groundwater. With injection of EOS®, TCE was effectively degraded to 42%–93% by the end of 175 days. The biostimulation with EOS® was useful in establishing a micro-site anaerobic but with limited contribution. Dilution of the aquifer movement also caused the TCE reduction among injection and monitoring wells. The degradability was affected by the location and the proximity from the injection well. TCE concentrations found to be negatively correlated with the associated Dehalococcoides spp. and functional genes levels. Dhc concentration of 108 copies L−1 caused the initial 40% of TCE degradation. The well with the optimal degradation owned tceA of 109 cells L−1. T-RFLP results indicate the wells with the superior TCE degradability also performed the highest Shannon index number (means the highest diversity), which occurred on the same day that Dhc levels started to enlarge. Desulfovibrio desulfuricans and Desulfuromonas chloroethenica were predominant species identified in the T-RFLP fingerprint profile. In brief, a variety of different factors including well locations, geochemical indicators, and microbial contribution were useful to explain the site-specific optimal TCE remediation approach. The consistence among TCE degradation, Dhc growing pattern, functional gene levels, and the dynamics of the microbial community structure present the novelty of this study.

原文English
頁(從 - 到)697-704
頁數8
期刊Chemosphere
233
DOIs
出版狀態Published - 2019 十月

指紋

Trichloroethylene
Environmental Monitoring
trichloroethylene
Remediation
remediation
Monitoring
monitoring
Groundwater
well
EOS
Degradation
Restriction Fragment Length Polymorphisms
degradation
Injections
Desulfuromonas
Desulfovibrio desulfuricans
Genes
Carcinogens
Environmental Biodegradation
groundwater

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

引用此文

Wu, Yi Ju ; Liu, Pao Wen Grace ; Hsu, You Siang ; Whang, Liang Ming ; Lin, Tsair Fuh ; Hung, Wei Nung ; Cho, Kun Ching. / Application of molecular biological tools for monitoring efficiency of trichloroethylene remediation. 於: Chemosphere. 2019 ; 卷 233. 頁 697-704.
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abstract = "Trichloroethylene (TCE) is one of the most ubiquitous halogenated organic compounds of concerns of carcinogens in groundwater in Taiwan. Bioremediation has been recognized as a cost-effective approach in reducing TCE concentration. Five pilot-scale wells were constructed to monitor TCE concentrations in contaminated groundwater. With injection of EOS{\circledR}, TCE was effectively degraded to 42{\%}–93{\%} by the end of 175 days. The biostimulation with EOS{\circledR} was useful in establishing a micro-site anaerobic but with limited contribution. Dilution of the aquifer movement also caused the TCE reduction among injection and monitoring wells. The degradability was affected by the location and the proximity from the injection well. TCE concentrations found to be negatively correlated with the associated Dehalococcoides spp. and functional genes levels. Dhc concentration of 108 copies L−1 caused the initial 40{\%} of TCE degradation. The well with the optimal degradation owned tceA of 109 cells L−1. T-RFLP results indicate the wells with the superior TCE degradability also performed the highest Shannon index number (means the highest diversity), which occurred on the same day that Dhc levels started to enlarge. Desulfovibrio desulfuricans and Desulfuromonas chloroethenica were predominant species identified in the T-RFLP fingerprint profile. In brief, a variety of different factors including well locations, geochemical indicators, and microbial contribution were useful to explain the site-specific optimal TCE remediation approach. The consistence among TCE degradation, Dhc growing pattern, functional gene levels, and the dynamics of the microbial community structure present the novelty of this study.",
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Application of molecular biological tools for monitoring efficiency of trichloroethylene remediation. / Wu, Yi Ju; Liu, Pao Wen Grace; Hsu, You Siang; Whang, Liang Ming; Lin, Tsair Fuh; Hung, Wei Nung; Cho, Kun Ching.

於: Chemosphere, 卷 233, 10.2019, p. 697-704.

研究成果: Article

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AU - Liu, Pao Wen Grace

AU - Hsu, You Siang

AU - Whang, Liang Ming

AU - Lin, Tsair Fuh

AU - Hung, Wei Nung

AU - Cho, Kun Ching

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AB - Trichloroethylene (TCE) is one of the most ubiquitous halogenated organic compounds of concerns of carcinogens in groundwater in Taiwan. Bioremediation has been recognized as a cost-effective approach in reducing TCE concentration. Five pilot-scale wells were constructed to monitor TCE concentrations in contaminated groundwater. With injection of EOS®, TCE was effectively degraded to 42%–93% by the end of 175 days. The biostimulation with EOS® was useful in establishing a micro-site anaerobic but with limited contribution. Dilution of the aquifer movement also caused the TCE reduction among injection and monitoring wells. The degradability was affected by the location and the proximity from the injection well. TCE concentrations found to be negatively correlated with the associated Dehalococcoides spp. and functional genes levels. Dhc concentration of 108 copies L−1 caused the initial 40% of TCE degradation. The well with the optimal degradation owned tceA of 109 cells L−1. T-RFLP results indicate the wells with the superior TCE degradability also performed the highest Shannon index number (means the highest diversity), which occurred on the same day that Dhc levels started to enlarge. Desulfovibrio desulfuricans and Desulfuromonas chloroethenica were predominant species identified in the T-RFLP fingerprint profile. In brief, a variety of different factors including well locations, geochemical indicators, and microbial contribution were useful to explain the site-specific optimal TCE remediation approach. The consistence among TCE degradation, Dhc growing pattern, functional gene levels, and the dynamics of the microbial community structure present the novelty of this study.

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