Application of protein phosphatase 1 inhibition assay and reversed phase liquid chromatography for the detection of microcystins in algal dietary supplements

Yi-Min Chen, Rang Huang, Hong Nong Chou

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

Protein phosphatase 1 inhibition assay and reversed phase high performance liquid chromatography were adjusted for the detection of microcystins in Chlorella and Spirulina dietary supplements. Various sample preparation procedures for the enzyme and chemical assays were tested by the toxin-spiked samples to improve the sensitivity and accuracy of these methods. A silica gel solid phase extraction coupled with a deactivated elution had remarkably reduced the matrix interference from sample extracts and improved the detection of microcystins in both assays. High performance liquid chromatography could give the composition of different microcystins in the sample if the resolution of separation and toxin standards were available. On the contrary, protein phosphatase 1 inhibition assay gave an accumulated inhibition activity of the sample that could be converted to MCYST-LR equivalent according to the IC50 of this standard. A good sensitivity of 50 ppb of microcystin in dried algal samples, was reached using the methods established in this research. Both enzyme and chemical assays were applied for the detection of possible microcystin contamination in the commercial products of algal dietary supplement. It was found that all nine samples of algal product examined in this study were free from microcystins.

原文English
頁(從 - 到)71-77
頁數7
期刊Journal of Food and Drug Analysis
13
發行號1
出版狀態Published - 2005 三月 1

All Science Journal Classification (ASJC) codes

  • 食品科學
  • 藥理

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