Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidus necator H16

Yoong Kit Leong, Pau Loke Show, John Chi Wei Lan, Rambabu Krishnamoorthy, Dinh Toi Chu, Dillirani Nagarajan, Hong Wei Yen, Jo-Shu Chang

研究成果: Article

摘要

Polyhydroxyalkanoates (PHAs), a family of biodegradable and renewable biopolymers show a huge potential as an alternative to conventional plastics. Extractive bioconversion (in situ product recovery)is a technique that integrates upstream fermentation and downstream purification. In this study, extractive bioconversion of PHAs from Cupriavidus necator H16 was performed via a thermo-separating aqueous two-phase system to reduce the cost and environmental impacts of PHAs production. Key operating parameters, such as polymer concentration, temperature, and pH, were optimized. The strategy achieved a yield and PF of 97.6% and 1.36-fold, respectively at 5% EOPO 3900 concentration, 30 °C fermentation temperature and pH 6. The PHAs production process was also successfully scaled up in a 2 L bioreactor. To the best of our knowledge, this is the first report on extractive fermentation of PHAs from Cupriavidus necator utilizing a thermo-separation system to achieve a better productivity and purity of the target product.

原文English
文章編號121474
期刊Bioresource technology
287
DOIs
出版狀態Published - 2019 九月 1

指紋

Polyhydroxyalkanoates
Bioconversion
Fermentation
fermentation
Biopolymers
Bioreactors
bioreactor
Purification
purification
Environmental impact
environmental impact
polymer
Productivity
plastic
temperature
Plastics
fold
Recovery
productivity
Temperature

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

引用此文

Leong, Yoong Kit ; Show, Pau Loke ; Lan, John Chi Wei ; Krishnamoorthy, Rambabu ; Chu, Dinh Toi ; Nagarajan, Dillirani ; Yen, Hong Wei ; Chang, Jo-Shu. / Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidus necator H16. 於: Bioresource technology. 2019 ; 卷 287.
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Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidus necator H16. / Leong, Yoong Kit; Show, Pau Loke; Lan, John Chi Wei; Krishnamoorthy, Rambabu; Chu, Dinh Toi; Nagarajan, Dillirani; Yen, Hong Wei; Chang, Jo-Shu.

於: Bioresource technology, 卷 287, 121474, 01.09.2019.

研究成果: Article

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AU - Krishnamoorthy, Rambabu

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AU - Chang, Jo-Shu

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