Application of virus-induced gene silencing technology in gene functional validation of orchids

Hsiang Chia Lu, Hong-Hwa Chen, Hsin Hung Yeh

研究成果: Chapter

4 引文 斯高帕斯(Scopus)

摘要

The largest family of angiosperms, Orchidaceae, has diverse, specialized pollination and ecological strategies and provides a rich subject for investigating evolutionary relationships and developmental biology. However, the study of these non-model organisms may be hindered by challenges, such as their large genome size, low transformation efficiency, long regeneration time, and long life-cycle. To overcome these obstacles, we first developed vectors with the use of a symptomless Cymbidium mosaic virus, which infects most orchids, then combined simple physiological controls and virus-induced gene silencing (VIGS) for validation of gene function in orchids. The success of our strategies was verified by our functional validation of floral identity gene(s) in the tetraploid Phalaenopsis orchids, which have an unusually long life-cycle (two years from sowing to flowering). We could knock down the RNA level of either a specific Phalaenopsis floral identity gene or a family of genes congruently in two months. Functional analysis of orchid genes could become easier and profit from the VIGS approach.

原文English
主出版物標題Orchid Biotechnology
發行者World Scientific Publishing Co.
頁面211-230
頁數20
ISBN(電子)9789812775900
ISBN(列印)9812706194, 9789812706195
DOIs
出版狀態Published - 2007 一月 1

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)

指紋

深入研究「Application of virus-induced gene silencing technology in gene functional validation of orchids」主題。共同形成了獨特的指紋。

引用此