Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy

Chia Wei Liu, Tien Chun Tsai, Masatoshi Osawa, Hsien Chang Chang, Ruey Jen Yang

研究成果: Article

9 引文 斯高帕斯(Scopus)

摘要

A sensing platform based on the attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique and immobilized aptamer has been proposed herein for the selective detection of mercury ions (Hg2+). In the proposed platform, 5′ thiolated 32-mer DNA probes with methylene blue at the 3′ end were immobilized on a thin gold (Au) surface layer. Following Hg2+ ions interacting with T bases of the aptamer, T-Hg-T bonds are formed; resulting in a hairpin-shaped formation of the DNA and a detectable change in the IR absorbance of the sensing interface. Notably, the background noise produced by external molecules (e.g., water, non-specific binding molecules and bulk solution) is reduced to a negligible level by means of the ATR detection mode. It is shown that the proposed sensor has a linear response (R2 = 0.986) with high sensitivity and good selectivity over the Hg2+ range of 0.01 μM–50 μM.

原文English
頁(從 - 到)137-147
頁數11
期刊Analytica Chimica Acta
1033
DOIs
出版狀態Published - 2018 十一月 29

    指紋

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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