@article{e014c96051ad478294c690220e7b3e24,
title = "Aptamer probed isolation of circulating tumor cells in cholangiocarcinoma patients",
abstract = "Cholangiocarcinoma (CCA) is a typically lethal, highly metastatic cancer which poses diagnostic challenges and is consequently associated with poor prognosis and high metastatic capability. Since the currently used diagnostic biomarkers are limited in sensitivity and specificity, probes with high-affinity and good specificity are needed to study metastasis in CCA. Circulating tumor cells (CTCs) in blood have been widely accepted as an indicator of diseases progression and metastasis in various other cancers. We hereby report the first use of a CCA specific aptamer probe, coupled on magnetic beads for the isolation of CCA CTCs from 35 advanced stage/metastatic patients on an integrated microfluidic platform with 100 % efficiency (>1 CTC/mL were isolated). The isolated cells were identified via immunofluorescence staining (CK+CD45−Hoechst+) and cell counting was assisted by ImageJ. The binding target of the aptamer on the CCA cancer cells was further analyzed and determined to be desmoplakin by liquid chromatography-mass spectrometry, western blotting, and immunofluorescence staining. It is envisioned that this biosensing platform could therefore be used for proactive CCA diagnostics in the near future.",
author = "Priya Gopinathan and Chiang, {Nai Jung} and Wang, {Chih Hung} and Anirban Sinha and Tsai, {Yi Cheng} and Tu, {Hsiu Chi} and Hung, {Shang Cheng} and Hsu, {Pang Hung} and Shan, {Yan Shen} and Lee, {Gwo Bin}",
note = "Funding Information: We would like to extend our thanks to the Mass Spectrometry Core Facility at Genomics Research Centre, Academia Sinica, Taiwan for performing mass spectrometry. The authors would also like to thank the National Health Research Institutes of Taiwan ( NHRI-EX108-10728EI to Y.S.S and G.B.L) and Taiwan{\textquoteright}s Ministry of Health and Welfare ( MOHW107-TDU-B-211-123003 to Y.S.S) for financial assistance. We also appreciate the partial financial support from the “Higher Education Support Project” of Taiwan{\textquoteright}s Ministry of Education (Grant No. 108Q2713E1 to G.B.L). The authors would also extend their thanks to the Ministry of Science and Technology of Taiwan ( MOST 106-2745-M-001-001-ASP , MOST 106-2113-M-259-009 , MOST-106-2113-M-001-009-MY2 to S.C.H and MOST 107-2221-E-007-013-MY3 to G.B.L) for financial support. Funding Information: YS Shan completed his MD in 1993. After the residency training in the Department of Surgery, National Cheng Kung University Hospital, Tainan, Taiwan, he got the PhD in 2004 from Institute of Clinical Medicine, National Cheng Kung University. He was pointed to establish and be the 1 st director of Division of Trauma (2011∼2016.7) and the 1 st UGI cancer team convener (2010∼2017 12) in National Cheng Kung University Hospital. Now, he is the distinguished professor of the Institute of Clinical Medicine, NCKU and Department of Surgery, NCKUH. He has published more than 160 papers in reputed journals and has been serving as several editorial board members of repute journals. Recent years, he devoted in the translational study of biliary tract cancer, pancreatic cancer, gastric cancer and GIST. He also performed several IIT and attended international trials in stomach, biliary, and pancreatic cancer. Since 2017 May, he also got the grant support from MOST to be the leader PI of TCTC-UGI group in Taiwan and was also nominated as member council of Asian-Ocean Pancreatic Association (AOPA) in 2018. From Aug 2019, he was elected as Dean of College of Medicine, NCKU. Funding Information: We would like to extend our thanks to the Mass Spectrometry Core Facility at Genomics Research Centre, Academia Sinica, Taiwan for performing mass spectrometry. The authors would also like to thank the National Health Research Institutes of Taiwan (NHRI-EX108-10728EI to Y.S.S and G.B.L) and Taiwan's Ministry of Health and Welfare (MOHW107-TDU-B-211-123003 to Y.S.S) for financial assistance. We also appreciate the partial financial support from the “Higher Education Support Project” of Taiwan's Ministry of Education (Grant No. 108Q2713E1 to G.B.L). The authors would also extend their thanks to the Ministry of Science and Technology of Taiwan (MOST 106-2745-M-001-001-ASP, MOST 106-2113-M-259-009, MOST-106-2113-M-001-009-MY2 to S.C.H and MOST 107-2221-E-007-013-MY3 to G.B.L) for financial support. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2020",
month = nov,
day = "1",
doi = "10.1016/j.snb.2020.128569",
language = "English",
volume = "322",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
}