AQU-1, a chromosomal class C β-lactamase, among clinical Aeromonas dhakensis isolates: Distribution and clinical significance

Chi Jung Wu, Hsuan Chen Wang, Po-Lin Chen, Ming Chung Chang, Hsiao-Fang Sun, Pei Hsin Chou, Wen-Chien Ko

研究成果: Article

5 引文 (Scopus)

摘要

Aeromonas dhakensis, a recently described Aeromonas sp. formerly called Aeromonas aquariorum, is associated with human infections. In this study, a chromosomal gene, bla AQU-1 , was identified in A. dhakensis AAK1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding CepH in Aeromonas hydrophila. An Escherichia coli TOP10 cell transformant harbouring bla AQU-1 was resistant to cefotaxime but not to cefepime. mRNA expression of bla AQU-1 in the cefotaxime-resistant mutant strain AAK1m was 70-fold higher than in the wild strain AAK1. In all 16 A. dhakensis isolates (the major species of 51 consecutive Aeromonas blood isolates collected from June 1999 to June 2001) as well as in A. aquariorum MDC47 T and A. hydrophila subsp. dhakensis LMG 19562 T , but not in the reference strains or clinical isolates of other A. hydrophila subspecies, Aeromonas caviae, Aeromonas veronii or Aeromonas enteropelogenes, bla AQU-1 -related genes were detected by PCR. Overall, 13 (81%) of the 16 A. dhakensis blood isolates exhibited either cefotaxime resistance or the in vitro emergence of derepressed cefotaxime-resistant mutants. In vivo selection of an A. dhakensis resistant mutant was noted in a burn patient undergoing cefotaxime monotherapy. These observations suggest that AQU-1 is a chromosomal cephalosporinase in A. dhakensis. Cefotaxime monotherapy for severe A. dhakensis infections should be used cautiously.

原文English
頁(從 - 到)456-461
頁數6
期刊International journal of antimicrobial agents
42
發行號5
DOIs
出版狀態Published - 2013 十一月 1

指紋

Hospital Distribution Systems
Aeromonas
Cefotaxime
Aeromonas hydrophila
Aeromonas caviae
Cephalosporinase
Genes
Infection
Open Reading Frames
Escherichia coli
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

引用此文

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title = "AQU-1, a chromosomal class C β-lactamase, among clinical Aeromonas dhakensis isolates: Distribution and clinical significance",
abstract = "Aeromonas dhakensis, a recently described Aeromonas sp. formerly called Aeromonas aquariorum, is associated with human infections. In this study, a chromosomal gene, bla AQU-1 , was identified in A. dhakensis AAK1 that constitutes a 1143-bp open reading frame and is 87{\%} identical to the gene encoding CepH in Aeromonas hydrophila. An Escherichia coli TOP10 cell transformant harbouring bla AQU-1 was resistant to cefotaxime but not to cefepime. mRNA expression of bla AQU-1 in the cefotaxime-resistant mutant strain AAK1m was 70-fold higher than in the wild strain AAK1. In all 16 A. dhakensis isolates (the major species of 51 consecutive Aeromonas blood isolates collected from June 1999 to June 2001) as well as in A. aquariorum MDC47 T and A. hydrophila subsp. dhakensis LMG 19562 T , but not in the reference strains or clinical isolates of other A. hydrophila subspecies, Aeromonas caviae, Aeromonas veronii or Aeromonas enteropelogenes, bla AQU-1 -related genes were detected by PCR. Overall, 13 (81{\%}) of the 16 A. dhakensis blood isolates exhibited either cefotaxime resistance or the in vitro emergence of derepressed cefotaxime-resistant mutants. In vivo selection of an A. dhakensis resistant mutant was noted in a burn patient undergoing cefotaxime monotherapy. These observations suggest that AQU-1 is a chromosomal cephalosporinase in A. dhakensis. Cefotaxime monotherapy for severe A. dhakensis infections should be used cautiously.",
author = "Wu, {Chi Jung} and Wang, {Hsuan Chen} and Po-Lin Chen and Chang, {Ming Chung} and Hsiao-Fang Sun and Chou, {Pei Hsin} and Wen-Chien Ko",
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T1 - AQU-1, a chromosomal class C β-lactamase, among clinical Aeromonas dhakensis isolates

T2 - Distribution and clinical significance

AU - Wu, Chi Jung

AU - Wang, Hsuan Chen

AU - Chen, Po-Lin

AU - Chang, Ming Chung

AU - Sun, Hsiao-Fang

AU - Chou, Pei Hsin

AU - Ko, Wen-Chien

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Aeromonas dhakensis, a recently described Aeromonas sp. formerly called Aeromonas aquariorum, is associated with human infections. In this study, a chromosomal gene, bla AQU-1 , was identified in A. dhakensis AAK1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding CepH in Aeromonas hydrophila. An Escherichia coli TOP10 cell transformant harbouring bla AQU-1 was resistant to cefotaxime but not to cefepime. mRNA expression of bla AQU-1 in the cefotaxime-resistant mutant strain AAK1m was 70-fold higher than in the wild strain AAK1. In all 16 A. dhakensis isolates (the major species of 51 consecutive Aeromonas blood isolates collected from June 1999 to June 2001) as well as in A. aquariorum MDC47 T and A. hydrophila subsp. dhakensis LMG 19562 T , but not in the reference strains or clinical isolates of other A. hydrophila subspecies, Aeromonas caviae, Aeromonas veronii or Aeromonas enteropelogenes, bla AQU-1 -related genes were detected by PCR. Overall, 13 (81%) of the 16 A. dhakensis blood isolates exhibited either cefotaxime resistance or the in vitro emergence of derepressed cefotaxime-resistant mutants. In vivo selection of an A. dhakensis resistant mutant was noted in a burn patient undergoing cefotaxime monotherapy. These observations suggest that AQU-1 is a chromosomal cephalosporinase in A. dhakensis. Cefotaxime monotherapy for severe A. dhakensis infections should be used cautiously.

AB - Aeromonas dhakensis, a recently described Aeromonas sp. formerly called Aeromonas aquariorum, is associated with human infections. In this study, a chromosomal gene, bla AQU-1 , was identified in A. dhakensis AAK1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding CepH in Aeromonas hydrophila. An Escherichia coli TOP10 cell transformant harbouring bla AQU-1 was resistant to cefotaxime but not to cefepime. mRNA expression of bla AQU-1 in the cefotaxime-resistant mutant strain AAK1m was 70-fold higher than in the wild strain AAK1. In all 16 A. dhakensis isolates (the major species of 51 consecutive Aeromonas blood isolates collected from June 1999 to June 2001) as well as in A. aquariorum MDC47 T and A. hydrophila subsp. dhakensis LMG 19562 T , but not in the reference strains or clinical isolates of other A. hydrophila subspecies, Aeromonas caviae, Aeromonas veronii or Aeromonas enteropelogenes, bla AQU-1 -related genes were detected by PCR. Overall, 13 (81%) of the 16 A. dhakensis blood isolates exhibited either cefotaxime resistance or the in vitro emergence of derepressed cefotaxime-resistant mutants. In vivo selection of an A. dhakensis resistant mutant was noted in a burn patient undergoing cefotaxime monotherapy. These observations suggest that AQU-1 is a chromosomal cephalosporinase in A. dhakensis. Cefotaxime monotherapy for severe A. dhakensis infections should be used cautiously.

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