Arsenic compounds induce apoptosis through caspase pathway activation in MA-10 leydig tumor cells

Yi Fen Mu, Ying Hui Chen, Ming Min Chang, Yung Chia Chen, Bu-Miin Huang

研究成果: Article

摘要

The incidence of testicular cancer is increasing worldwide. Leydig cell tumors represent one type of sex cord-stromal testis malignancy, which tend to respond unfavorably to chemotherapies. Identifying more efficient treatment strategies is therefore crucial for patients. The present study aimed to investigate the apoptotic effects of arsenic compounds and their underlying mechanisms. The results indicated that sodium arsenite and dimethylarsenic acid induced apoptosis of the murine Leydig tumor cell line, MA-10. These apoptotic effects were characterized morpho-logically by membrane blebbing and cell detachment assays, biochemically using a cell viability assay, and cytologically by flow cytometry analysis. Western blotting demonstrated that caspases-3, -8 and -9, and poly(ADP-ribose) polymerase protein levels were increased compared with untreated MA-10 cells; however, the caspase inhibitor, Z-VAD-fmk, reversed these effects. In conclusion, the present study has shown that sodium arsenite and dimethylarsenic acid may activate the intrinsic and extrinsic caspase pathways, and induce MA-10 cell apoptosis. These results suggest that sodium arsenite and dimethylarsenic acid may represent novel approaches to treat clinically unmanageable forms of testicular cancer.

原文English
頁(從 - 到)944-954
頁數11
期刊Oncology Letters
18
發行號1
DOIs
出版狀態Published - 2019 一月 1

指紋

Leydig Cell Tumor
Arsenicals
Caspases
Testicular Neoplasms
Apoptosis
Acids
Caspase Inhibitors
Poly(ADP-ribose) Polymerases
Caspase 8
Blister
Tumor Cell Line
Caspase 3
Testis
Cell Survival
Flow Cytometry
Western Blotting
Cell Membrane
Drug Therapy
Incidence
sodium arsenite

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

引用此文

Mu, Yi Fen ; Chen, Ying Hui ; Chang, Ming Min ; Chen, Yung Chia ; Huang, Bu-Miin. / Arsenic compounds induce apoptosis through caspase pathway activation in MA-10 leydig tumor cells. 於: Oncology Letters. 2019 ; 卷 18, 編號 1. 頁 944-954.
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abstract = "The incidence of testicular cancer is increasing worldwide. Leydig cell tumors represent one type of sex cord-stromal testis malignancy, which tend to respond unfavorably to chemotherapies. Identifying more efficient treatment strategies is therefore crucial for patients. The present study aimed to investigate the apoptotic effects of arsenic compounds and their underlying mechanisms. The results indicated that sodium arsenite and dimethylarsenic acid induced apoptosis of the murine Leydig tumor cell line, MA-10. These apoptotic effects were characterized morpho-logically by membrane blebbing and cell detachment assays, biochemically using a cell viability assay, and cytologically by flow cytometry analysis. Western blotting demonstrated that caspases-3, -8 and -9, and poly(ADP-ribose) polymerase protein levels were increased compared with untreated MA-10 cells; however, the caspase inhibitor, Z-VAD-fmk, reversed these effects. In conclusion, the present study has shown that sodium arsenite and dimethylarsenic acid may activate the intrinsic and extrinsic caspase pathways, and induce MA-10 cell apoptosis. These results suggest that sodium arsenite and dimethylarsenic acid may represent novel approaches to treat clinically unmanageable forms of testicular cancer.",
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Arsenic compounds induce apoptosis through caspase pathway activation in MA-10 leydig tumor cells. / Mu, Yi Fen; Chen, Ying Hui; Chang, Ming Min; Chen, Yung Chia; Huang, Bu-Miin.

於: Oncology Letters, 卷 18, 編號 1, 01.01.2019, p. 944-954.

研究成果: Article

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