Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity

Michael T. Yang, Jianping Fu, Yang Kao Wang, Ravi A. Desai, Christopher S. Chen

研究成果: Article同行評審

187 引文 斯高帕斯(Scopus)

摘要

We describe the use of a microfabricated cell culture substrate, consisting of a uniform array of closely spaced, vertical, elastomeric microposts, to study the effects of substrate rigidity on cell function. Elastomeric micropost substrates are micromolded from silicon masters comprised of microposts of different heights to yield substrates of different rigidities. The tips of the elastomeric microposts are functionalized with extracellular matrix through microcontact printing to promote cell adhesion. These substrates, therefore, present the same topographical cues to adherent cells while varying substrate rigidity only through manipulation of micropost height. This protocol describes how to fabricate the silicon micropost array masters (∼2 weeks to complete) and elastomeric substrates (3 d), as well as how to perform cell culture experiments (1-14 d), immunofluorescence imaging (2 d), traction force analysis (2 d) and stem cell differentiation assays (1 d) on these substrates in order to examine the effect of substrate rigidity on stem cell morphology, traction force generation, focal adhesion organization and differentiation.

原文English
頁(從 - 到)187-213
頁數27
期刊Nature Protocols
6
發行號2
DOIs
出版狀態Published - 2011 二月

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)

指紋

深入研究「Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity」主題。共同形成了獨特的指紋。

引用此