Assays for angiotensin converting enzyme inhibitory activity

Bin Wha Chang, Richie L.C. Chen, I. Jen Huang, Hsien Chang Chang

研究成果: Article同行評審

44 引文 斯高帕斯(Scopus)

摘要

A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.

原文English
頁(從 - 到)84-88
頁數5
期刊Analytical Biochemistry
291
發行號1
DOIs
出版狀態Published - 2001 4月 1

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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