摘要
A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.
原文 | English |
---|---|
頁(從 - 到) | 84-88 |
頁數 | 5 |
期刊 | Analytical Biochemistry |
卷 | 291 |
發行號 | 1 |
DOIs | |
出版狀態 | Published - 2001 4月 1 |
All Science Journal Classification (ASJC) codes
- 生物物理學
- 生物化學
- 分子生物學
- 細胞生物學