Assessing the fate, toxicity, and ecological risk of mixtures of di(2-ethylhexyl)phthalate and di-n-butylphthalate using aquatic and terrestrial microcosms

Di(2-ethylhexyl)phthalate (DEHP) and di-n-butylphthalate (DBP) frequently coexist in different environmental compartments. Thus, in this study, model aquatic and terrestrial microcosms were prepared to analyze the combined effect of DEHP and DBP on their fate, toxicity, and ecological risk. In the aquatic microcosms, with the addition of the same amount of DEHP and DBP, a higher total amount of DEHP was detected in water, suspended particles, and sediment than DBP due to the higher K_ow and half-life of DEHP than DBP. Sediment was the major sink of both phthalates, as the highest percentages of DEHP (90.0 % ∼ 95.6 %) and DBP (68.7 % ∼ 78.1 %) were found in the sediment. The results of the whole sediment toxicity test showed that DBP (LC₅₀/LC₁₀: 6.75/1.171 μg/g dw) was more toxic than DEHP (LC₅₀/LC₁₀: 158.75/27.25 μg/g dw) to the tubificid oligochaete Monopylephorus limosus, with a synergistic toxic effect of the mixture of DEHP and DBP (LC₅₀/LC₁₀: 100.3/4.6 μg/g dw). The mobility of DEHP and DBP in soil was low during irrigation, with the release of 0.054 % ∼ 2.29 % DEHP and 0.097 % ∼ 1.86 % DBP. The bioconcentration factors/biota–sediment accumulation factors for DEHP (70.8–145 L/kg/0.093–0.359) in the muscle of the fish Carassius auratus were lower than those for DBP (82.2–300 L/kg/0.514–1.625). The bioaccumulation factors of DEHP and DBP for earthworms were 0.373 and 0.682, respectively. The levels of DEHP and DBP in the water and sediment of aquatic systems and in the soil of terrestrial systems might pose high ecological risks to some fish species, M. limosus and earthworms, according to the risk quotient values. These data provide valuable insights for the development of government control strategies to minimize the ecological risks of DEHP and DBP.