Atrial Identity Is Determined by a COUP-TFII Regulatory Network

San pin Wu, Chiang Min Cheng, Rainer B. Lanz, Tiannan Wang, Jonathan L. Respress, Sameer Ather, Wen Chen, Shaw Jenq Tsai, Xander H.T. Wehrens, Ming Jer Tsai, Sophia Y. Tsai

研究成果: Article同行評審

65 引文 斯高帕斯(Scopus)

摘要

Atria and ventricles exhibit distinct molecular profiles that produce structural and functional differences between the two cardiac compartments. However, the factors that determine these differences remain largely undefined. Cardiomyocyte-specific COUP-TFII ablation produces ventricularized atria that exhibit ventricle-like action potentials, increased cardiomyocyte size, and development of extensive T tubules. Changes in atrial characteristics are accompanied by alterations of 2,584 genes, of which 81% were differentially expressed between atria and ventricles, suggesting that a major function of myocardial COUP-TFII is to determine atrial identity. Chromatin immunoprecipitation assays using E13.5 atria identified classic atrial-ventricular identity genes Tbx5, Hey2, Irx4, MLC2v, MLC2a, and MLC1a, among many other cardiac genes, as potential COUP-TFII direct targets. Collectively, our results reveal that COUP-TFII confers atrial identity through direct binding and by modulating expression of a broad spectrum of genes that have an impact on atrial development and function.

原文English
頁(從 - 到)417-426
頁數10
期刊Developmental Cell
25
發行號4
DOIs
出版狀態Published - 2013 五月 28

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 生物化學、遺傳與分子生物學 (全部)
  • 發展生物學
  • 細胞生物學

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