Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis

D. R. Meldrum, W. H. Pence, S. E. Moody, D. L. Cunningham, M. Holl, P. J. Wiktor, M. Saini, M. P. Moore, L. S. Jang, M. Kidd, C. Fisher, A. Cookson

研究成果: Paper

9 引文 (Scopus)

摘要

Over the past 5 years the Genomation Laboratory at the University of Washington has developed an automated fluid handling system called "Acapella" to prepare microliter reactions for genome analysis. Reactions such as restriction enzyme digests, polymerase chain reactions (PCRs), and sequencing reactions are prepared in glass capillaries, one per sample, with an automated system that can process 5,000 samples in 8 hours. A DNA sample is aspirated from a microplate well with a novel piezoelectric aspirator/mixer actuator, reagents are dispensed into the end of the capillary with piezoelectric reagent dispensers, all fluids inside the capillary are mixed with the piezoelectric aspirator/mixer actuator, and the capillary is off-loaded to a capillary cassette for further processing. On-going development includes new, automated modules for thermal processing of capillaries, real-time DNA quantitation, and purification of DNA inside of capillaries to prepare the samples for DNA sequencing. Applicatio ns of the technology include minimal residual disease quantification and sample preparation for DNA sequencing.

原文English
頁面1211-1219
頁數9
出版狀態Published - 2001 一月 1
事件2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings - Como, Italy
持續時間: 2001 七月 82001 七月 12

Other

Other2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings
國家Italy
城市Como
期間01-07-0801-07-12

指紋

Thermal cycling
Purification
DNA
Throughput
Fluids
Actuators
Dispensers
Polymerase chain reaction
Enzymes
Genes
Glass
Processing

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Software
  • Computer Science Applications
  • Electrical and Electronic Engineering

引用此文

Meldrum, D. R., Pence, W. H., Moody, S. E., Cunningham, D. L., Holl, M., Wiktor, P. J., ... Cookson, A. (2001). Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis. 1211-1219. 論文發表於 2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings, Como, Italy.
Meldrum, D. R. ; Pence, W. H. ; Moody, S. E. ; Cunningham, D. L. ; Holl, M. ; Wiktor, P. J. ; Saini, M. ; Moore, M. P. ; Jang, L. S. ; Kidd, M. ; Fisher, C. ; Cookson, A. / Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis. 論文發表於 2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings, Como, Italy.9 p.
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Meldrum, DR, Pence, WH, Moody, SE, Cunningham, DL, Holl, M, Wiktor, PJ, Saini, M, Moore, MP, Jang, LS, Kidd, M, Fisher, C & Cookson, A 2001, 'Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis', 論文發表於 2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings, Como, Italy, 01-07-08 - 01-07-12 頁 1211-1219.

Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis. / Meldrum, D. R.; Pence, W. H.; Moody, S. E.; Cunningham, D. L.; Holl, M.; Wiktor, P. J.; Saini, M.; Moore, M. P.; Jang, L. S.; Kidd, M.; Fisher, C.; Cookson, A.

2001. 1211-1219 論文發表於 2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings, Como, Italy.

研究成果: Paper

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AU - Wiktor, P. J.

AU - Saini, M.

AU - Moore, M. P.

AU - Jang, L. S.

AU - Kidd, M.

AU - Fisher, C.

AU - Cookson, A.

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Meldrum DR, Pence WH, Moody SE, Cunningham DL, Holl M, Wiktor PJ 等. Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis. 2001. 論文發表於 2001 IEEE/ASME International Conference on Advanced Intelligent Mechatronics Proceedings, Como, Italy.