Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization

Yan Wei Wu, Clément Mettling, Shang Rung Wu, Chia Yi Yu, Guey Chuen Perng, Yee Shin Lin, Yea Lih Lin

研究成果: Article

16 引文 (Scopus)

摘要

One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA can be protected within the autophagic organelles since anti-dengue neutralizing antibodies do not have an effect on the vesicle-mediated transmission that is able to initiate a new round of infection in target cells. Importantly, such infectious vesicles were also detected in a patient serum. Our study suggests that autophagy machinery plays a new role in dengue virus transmission. This discovery explains the inefficiency of neutralizing antibody upon dengue infection as a potential immune evasion mechanism in vivo.

原文English
文章編號32243
期刊Scientific reports
6
DOIs
出版狀態Published - 2016 八月 25

指紋

Dengue
Autophagy
Neutralizing Antibodies
Viral RNA
Antibodies
Immune Evasion
Dengue Virus
Viral Proteins
Infection
Organelles
Viruses
Serum

All Science Journal Classification (ASJC) codes

  • General

引用此文

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abstract = "One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA can be protected within the autophagic organelles since anti-dengue neutralizing antibodies do not have an effect on the vesicle-mediated transmission that is able to initiate a new round of infection in target cells. Importantly, such infectious vesicles were also detected in a patient serum. Our study suggests that autophagy machinery plays a new role in dengue virus transmission. This discovery explains the inefficiency of neutralizing antibody upon dengue infection as a potential immune evasion mechanism in vivo.",
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AU - Mettling, Clément

AU - Wu, Shang Rung

AU - Yu, Chia Yi

AU - Perng, Guey Chuen

AU - Lin, Yee Shin

AU - Lin, Yea Lih

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