Azetidine-induced accumulation of class i small heat shock proteins in the soluble fraction provides thermotolerance in soybean seedlings

Tsung Luo Jinn, Chi Chou Chiu, Wei Wen Song, Yih Ming Chen, Chu Yung Lin

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40°C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45°C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance.

原文English
頁(從 - 到)1759-1767
頁數9
期刊Plant and Cell Physiology
45
發行號12
DOIs
出版狀態Published - 2004 12月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學

指紋

深入研究「Azetidine-induced accumulation of class i small heat shock proteins in the soluble fraction provides thermotolerance in soybean seedlings」主題。共同形成了獨特的指紋。

引用此