Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan

Chi Jung Wu, Yin Ching Chuang, Mei Feng Lee, Chin Chi Lee, Hsin Chun Lee, Nan Yao Lee, Chia Ming Chang, Po Lin Chen, Yu Tzu Lin, Jing Jou Yan, Wen Chien Ko

研究成果: Article

32 引文 (Scopus)

摘要

Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6%) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.

原文English
頁(從 - 到)5813-5818
頁數6
期刊Antimicrobial agents and chemotherapy
55
發行號12
DOIs
出版狀態Published - 2011 十二月 1

指紋

Aeromonas
Bacteremia
Taiwan
Aeromonas caviae
Clavulanic Acid
Genes
Disk Diffusion Antimicrobial Tests
Aeromonas hydrophila
Phenotype
Phlebitis
Lactams
Cefotaxime
Sequence Analysis
Plasmids
Catheters
Chromosomes
Polymerase Chain Reaction
Infection

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

引用此文

Wu, Chi Jung ; Chuang, Yin Ching ; Lee, Mei Feng ; Lee, Chin Chi ; Lee, Hsin Chun ; Lee, Nan Yao ; Chang, Chia Ming ; Chen, Po Lin ; Lin, Yu Tzu ; Yan, Jing Jou ; Ko, Wen Chien. / Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan. 於: Antimicrobial agents and chemotherapy. 2011 ; 卷 55, 編號 12. 頁 5813-5818.
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title = "Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan",
abstract = "Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6{\%}) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.",
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Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan. / Wu, Chi Jung; Chuang, Yin Ching; Lee, Mei Feng; Lee, Chin Chi; Lee, Hsin Chun; Lee, Nan Yao; Chang, Chia Ming; Chen, Po Lin; Lin, Yu Tzu; Yan, Jing Jou; Ko, Wen Chien.

於: Antimicrobial agents and chemotherapy, 卷 55, 編號 12, 01.12.2011, p. 5813-5818.

研究成果: Article

TY - JOUR

T1 - Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan

AU - Wu, Chi Jung

AU - Chuang, Yin Ching

AU - Lee, Mei Feng

AU - Lee, Chin Chi

AU - Lee, Hsin Chun

AU - Lee, Nan Yao

AU - Chang, Chia Ming

AU - Chen, Po Lin

AU - Lin, Yu Tzu

AU - Yan, Jing Jou

AU - Ko, Wen Chien

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6%) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.

AB - Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6%) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.

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