Baculovirus as versatile vectors for protein display and biotechnological applications

Chih Hsuan Tsai, Sung Chan Wei, Huei Ru Lo, Yu Chan Chao

研究成果: Article同行評審

26 引文 斯高帕斯(Scopus)

摘要

The baculovirus–insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production. Apart from the advantage of large-scale heterologous protein production with appropriate eukaryotic post-translational modification, foreign proteins can also be displayed on the viral envelope. This surface-display technology preserves the native multimeric structure of the protein, thereby expanding the clinical and pharmaceutical utility of the baculovirus system. Recombinant baculoviruses displaying major antigens for human or animal viruses can serve as appropriate vaccines. They can also serve as effective diagnostic platforms and various cell-based assay systems. In this review, we discuss progress in applying baculovirus surface-display, including protein display on the envelope, capsid, and occlusion bodies of baculoviruses, as well as on cells. We will also describe strategies for improvement of this biotechnological approach.

原文English
頁(從 - 到)231-255
頁數25
期刊Current issues in molecular biology
34
DOIs
出版狀態Published - 2020

All Science Journal Classification (ASJC) codes

  • 微生物學
  • 分子生物學
  • 微生物學(醫學)

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