Balanophora spicata and lupeol acetate possess antinociceptive and anti-inflammatory activities in vivo and in vitro

Yuh Fung Chen, Chien Ching, Tian Shung Wu, Chi Rei Wu, Wen Tsong Hsieh, Huei Yann Tsai

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

Aims of the present study were to investigate effects of Balanophora spicata (BS) on antinociception and anti-inflammation both in vivo and in vitro. Crude extract of BS inhibited vascular permeability induced by histamine, serotonin, bradykinin, and PGE but not by PAF. Furthermore, BS crude extract, different layers (n-hexane, ethyl acetate, n-butanol, and water layer), and lupeol acetate had significant antinociceptive and anti-inflammatory effects on acetic acid-induced abdominal writhing response, formalin-induced licking behavior, carrageenan-, and serotonin-induced paw edema. The n-hexane layer had the most effective potency among all layers (IC50: 67.33 mg/kg on writhing response; IC50s: 34.2 mg/kg and 21.29 mg/kg on the early phase and late phase of formalin test, resp.). Additionally, lupeol acetate which was isolated from the n-hexane layer of BS effectively inhibited the acetic acid-induced writhing response (IC50: 28.32 mg/kg), formalin-induced licking behavior (IC50: 20.95 mg/kg), NO production (IC50: 4.102 M), iNOS expression (IC50: 5.35 M), and COX2 expression (IC50: 5.13 M) in LPS-stimulated RAW 264.7 cells. In conclusion, BS has antinociceptive and anti-inflammatory effects which may be partially due to the inhibition of changes in vascular permeability induced by histamine, serotonin, bradykinin, and PGEand the attenuation of iNOS and COX-2 expression.

原文English
文章編號371273
期刊Evidence-based Complementary and Alternative Medicine
2012
DOIs
出版狀態Published - 2012

All Science Journal Classification (ASJC) codes

  • 補充和替代醫學

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