Barrier disruption stimulates interleukin-1α expression and release from a pre-formed pool in murine epidermis

Ladonna C. Wood, Peter M. Elias, Cornelia Calhoun, Janice C. Tsai, Carl Grunfeld, Kenneth R. Feingold

研究成果: Article

220 引文 斯高帕斯(Scopus)


Previous studies have shown that barrier disruption increases epidermal mRNA levels of interleukin-1α (IL-1α). We used immunohistochemistry to examine IL-1α expression in hairless mouse skin under basal conditions and following barrier abrogation. In untreated mice, IL-1α was present in the dermis and nucleated epidermal layers in a diffuse, generalized pattern. In essential fatty acid deficient mice IL-1α was present in all epidermal layers and the dermis, with prominent staining in the stratum corneum. After acute barrier disruption with tape-stripping, IL-1α increased in the epidermis and dermis within 10 min, remained elevated at 2 and 4 h, and decreased to near basal levels by 24 h. Moreover, intense, perinuclear, basal cell staining appeared at 10 min, persisting until 4 h after barrier disruption. Since the increase in IL-1α immunostaining after acute barrier abrogation precedes the increase in mRNA, we hypothesized that the IL-1α might derive from a preformed pool. Prolonged occlusion of normal skin, a treatment that specifically reduces epidermal mRNA levels of IL-1α, decreased basal immunostaining for IL-1α and blunted the increase in IL-1α usually seen following barrier disruption. Moreover, tape-stripping of skin, maintained ex vivo at 4°C, resulted in increased IL-1α immunostaining within the upper nucleated epidermal layers, as well as release of mature IL-1α into the medium, as measured by Western blotting and enzyme-linked immunosorbent assay. In addition, the stratum corneum attached to the tape contained IL-1α. These studies show that acute barrier disruption induces both the immediate release and dispersion of IL-1α from a pre-formed, epidermal pool, as well as increased IL-1α synthesis; both mechanisms are consistent with a role for IL-1α in the regulation of proinflammatory and homeostatic processes in the skin.

頁(從 - 到)397-403
期刊Journal of Investigative Dermatology
出版狀態Published - 1996 一月 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

指紋 深入研究「Barrier disruption stimulates interleukin-1α expression and release from a pre-formed pool in murine epidermis」主題。共同形成了獨特的指紋。

  • 引用此