Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation

Hsi Hui Lin, Tzi Peng Yang, Si Tse Jiang, Hsi Yuan Yang, Ming Jer Tang

研究成果: Article同行評審

71 引文 斯高帕斯(Scopus)

摘要

Background. Madin-Darby canine kidney (MDCK) cells develop into simple epithelial cell cysts when cultured in type I collagen gel. We found that MDCK cells initially grow into multilayer cell aggregates and subsequently develop central lumen that contain apoptotic cells. We hypothesized that apoptosis might be essential for the formation of MDCK cysts. Methods. Using MDCK cells cultured in collagen gel as the experimental model, we investigated how renal cells organize to form cysts. To delineate the role of apoptosis in the process of cyst formation, MDCK cells were transfected with the bcl-2 gene. Characterization of apoptosis was studied by morphological and biochemical methods. Results. Bcl-2 overexpression conferred resistance to apoptosis. Cultured in collagen gel, Bcl-2 transfectants rarely formed a simple epithelial cyst, but instead remained as a multilayer cell aggregate containing central or multiple lumens, or even developing into branching structures. Conclusions. Because Bcl-2 overexpression averts cyst cavitation, these data clearly indicate that apoptosis is an essential initial event for renal cyst formation.

原文English
頁(從 - 到)168-178
頁數11
期刊Kidney international
55
發行號1
DOIs
出版狀態Published - 1999 一月 1

All Science Journal Classification (ASJC) codes

  • Nephrology

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