TY - JOUR
T1 - Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia
AU - Lien, Kang Yi
AU - Liu, Chien Ju
AU - Kuo, Pao-Lin
AU - Lee, Gwo Bin
PY - 2009/6/1
Y1 - 2009/6/1
N2 - This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.
AB - This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.
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U2 - 10.1109/MEMSYS.2009.4805388
DO - 10.1109/MEMSYS.2009.4805388
M3 - Conference article
AN - SCOPUS:65949110604
SN - 1084-6999
SP - 340
EP - 343
JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
M1 - 4805388
T2 - 22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009
Y2 - 25 January 2009 through 29 January 2009
ER -