Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

Kang Yi Lien, Chien Ju Liu, Pao-Lin Kuo, Gwo Bin Lee

研究成果: Conference article同行評審

1 引文 斯高帕斯(Scopus)

摘要

This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.

原文English
文章編號4805388
頁(從 - 到)340-343
頁數4
期刊Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs
出版狀態Published - 2009 6月 1
事件22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy
持續時間: 2009 1月 252009 1月 29

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 機械工業
  • 電氣與電子工程

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