TY - GEN
T1 - Bead-based miniature microfluidic systems for rapid RNA extraction and reverse transcription
AU - Lien, Kang Yi
AU - Liu, Chien Ju
AU - Shin, Jyh Wei
AU - Chang, Tsuey Yu
AU - Lee, Gwo Bin
PY - 2008
Y1 - 2008
N2 - The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bioseparators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.
AB - The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bioseparators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.
UR - http://www.scopus.com/inward/record.url?scp=49449100104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=49449100104&partnerID=8YFLogxK
U2 - 10.1115/MNHT2008-52268
DO - 10.1115/MNHT2008-52268
M3 - Conference contribution
AN - SCOPUS:49449100104
SN - 0791842924
SN - 9780791842928
T3 - 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
SP - 251
EP - 256
BT - 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
T2 - 1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08
Y2 - 6 January 2008 through 9 January 2008
ER -