Bead-based miniature microfluidic systems for rapid RNA extraction and reverse transcription

Kang Yi Lien, Chien Ju Liu, Jyh Wei Shin, Tsuey Yu Chang, Gwo Bin Lee

研究成果: Conference contribution

摘要

The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bioseparators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.

原文English
主出版物標題2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
頁面251-256
頁數6
DOIs
出版狀態Published - 2008 八月 20
事件1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08 - Tainan, Taiwan
持續時間: 2008 一月 62008 一月 9

出版系列

名字2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
PART A

Other

Other1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08
國家Taiwan
城市Tainan
期間08-01-0608-01-09

All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Materials Science(all)
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics

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  • 引用此

    Lien, K. Y., Liu, C. J., Shin, J. W., Chang, T. Y., & Lee, G. B. (2008). Bead-based miniature microfluidic systems for rapid RNA extraction and reverse transcription. 於 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008 (頁 251-256). (2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008; 卷 PART A). https://doi.org/10.1115/MNHT2008-52268