Berberine reduces Toll-like receptor-mediated macrophage migration by suppression of Src enhancement

Wei Erh Cheng, Miao Ying Chang, Jyun Yan Wei, Yen Jen Chen, Ming Chei Maa, Tzeng Horng Leu

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

Berberine is an isoquinoline with anti-inflammatory activity. We previously demonstrated that there was a loop of signal amplification between nuclear factor kappa B and Src for macrophage mobility triggered by the engagement of Toll-like receptors (TLRs). The simultaneous suppression of lipopolysaccharide (LPS)-mediated upregulation of inducible nitric oxide synthase, cyclooxygenase 2, and cell mobility in berberine-treated macrophages suggested Src might be a target of berberine. Indeed, th reduced migration, greatly suppressed Src induction in both protein and RNA transcript by berberine were observed in macrophages exposed to LPS, peptidoglycan, polyinosinic-polycytidylic acid, and CpG-oligodeoxynucleotides. In addition to Src induction, berberine also inhibited LPS-mediated Src activation in Src overexpressing macrophages and S-nitroso-N-acetylpenicillamine (a nitric oxide donor) could partly restore it. Moreover, berberine suppressed Src activity in fibronectin-stimulated macrophages and in v-Src transformed cells. These results implied that by effectively reducing Src expression and activity, berberine inhibited TLR-mediated cell motility in macrophages.

原文English
頁(從 - 到)1-10
頁數10
期刊European Journal of Pharmacology
757
DOIs
出版狀態Published - 2015 六月 15

All Science Journal Classification (ASJC) codes

  • Pharmacology

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