Beyond the Debye length in high ionic strength solution: Direct protein detection with field-effect transistors (FETs) in human serum

Chia Ho Chu, Indu Sarangadharan, Abiral Regmi, Yen Wen Chen, Chen Pin Hsu, Wen Hsin Chang, Geng Yen Lee, Jen Inn Chyi, Chih Chen Chen, Shu Chu Shiesh, Gwo Bin Lee, Yu Lin Wang

研究成果: Article

72 引文 斯高帕斯(Scopus)

摘要

In this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) are used to directly detect proteins, including HIV-1 RT, CEA, NT-proBNP and CRP, in 1X PBS (with 1%BSA) or human sera. The samples do not need any dilution or washing process to reduce the ionic strength. The sensor shows high sensitivity and the detection takes only 5 minutes. The designs of the sensor, the methodology of the measurement, and the working mechanism of the sensor are discussed and investigated. A theoretical model is proposed based on the finding of the experiments. This sensor is promising for point-of-care, home healthcare, and mobile diagnostic device.

原文English
文章編號5256
期刊Scientific reports
7
發行號1
DOIs
出版狀態Published - 2017 十二月 1

All Science Journal Classification (ASJC) codes

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    Chu, C. H., Sarangadharan, I., Regmi, A., Chen, Y. W., Hsu, C. P., Chang, W. H., Lee, G. Y., Chyi, J. I., Chen, C. C., Shiesh, S. C., Lee, G. B., & Wang, Y. L. (2017). Beyond the Debye length in high ionic strength solution: Direct protein detection with field-effect transistors (FETs) in human serum. Scientific reports, 7(1), [5256]. https://doi.org/10.1038/s41598-017-05426-6