Binary combinatorial scanning reveals potent poly-alanine-substituted inhibitors of protein-protein interactions

Xiyun Ye, Yen Chun Lee, Zachary P. Gates, Yingjie Ling, Jennifer C. Mortensen, Fan Shen Yang, Yu Shan Lin, Bradley L. Pentelute

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Establishing structure–activity relationships is crucial to understand and optimize the activity of peptide-based inhibitors of protein–protein interactions. Single alanine substitutions provide limited information on the residues that tolerate simultaneous modifications with retention of biological activity. To guide optimization of peptide binders, we use combinatorial peptide libraries of over 4,000 variants—in which each position is varied with either the wild-type residue or alanine—with a label-free affinity selection platform to study protein–ligand interactions. Applying this platform to a peptide binder to the oncogenic protein MDM2, several multi-alanine-substituted analogs with picomolar binding affinity were discovered. We reveal a non-additive substitution pattern in the selected sequences. The alanine substitution tolerances for peptide ligands of the 12ca5 antibody and 14-3-3 regulatory protein are also characterized, demonstrating the general applicability of this new platform. We envision that binary combinatorial alanine scanning will be a powerful tool for investigating structure–activity relationships.

原文English
文章編號128
期刊Communications Chemistry
5
發行號1
DOIs
出版狀態Published - 2022 12月

All Science Journal Classification (ASJC) codes

  • 一般化學
  • 環境化學
  • 生物化學
  • 材料化學

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