Biopile study of the effects of bioaugmentation and biostimulation on bioremediation of TPH-contaminated soil

Po Tseng Pan, Sheng Shung Cheng, Chun Hsuan Gaop, Tsung Lin Hsieh, Li Hsin Chang, Liang Ming Whang, Tsung Chung Chang, Pao Wen Liu

研究成果: Conference contribution

摘要

Contamination of total petroleum hydrocarbons (TPH) has caused critical environmental and health defects. Laboratory microcosms were used to measure the change in TPH concentration and the accompanying changes in microbial diversity with time. During the experiments, soil samples were collected and analyzed periodically to determine the amount of residual TPH and evaluate the effects of amendments on microbial diversity. A recently TPH-contaminated soil site has investigated to apply different enhanced bioremediation process with lab-scale of 0.0015m3 biopiles. Conceptual design of five biopiles was conducted to provide: (1).non-agitation biopile with the indigenous microbes as Natural Attenuation test, NA, (2).well-mixing biopile with the indigenous microbes as control test, CT, (3).bioaugmentation with five types of enrichment culture of TPH-degrading bacteria, BAT, (4).biostimulation with biosurfactant (rhamnolipid) to emulsify the entrapped diesel adsorbed onto the soil pore, BS. (5).combined bioaugmentation and biostimulation with TPH-degrading bacteria and biosurfactant as BAS test. Besides NA biopile, periodic land-farming procedure was employed to these four biopiles(CTinverted commas BATinverted commas BSinverted commas BAS). Weekly turn over mixing and water spray could keep 0.0015m3 of loamy sand soil with enough oxygen content above 10%-15% and moisture about 20%. The original total petroleum hydrocarbon (TPH C10-C40) was about 1,4032 mg TPH/ kg dry soil and the total indigenous microbial concentration was measured at the range of 3.0×104 CFU/g soil that meaned the biodegradation would be happened. Therefore, the TPH removal efficiencies (Day 112) achieved at different levels in these five biopiles, NA=24.8%, CT=62.7%, BAT=67.2%, BS=77.2%, BAS=72.7%. Bioremediation performance was promoted by the bioaugmentation and the biosurfactant addition (BS). Before 35 days, the first order reaction rate constant K values (day-1) was evaluated as BAS(0.0329)> BAT(0.0189) >BS(0.0151)>CT(0.0095)>NA(0.0009). However, the slowing of degradation rate during the later stage could be attributed to the properties of the remaining hydrocarbons and environmental conditions. This increase of polar fraction to the results of biomass formation associated with the biodegration of hydrocarbons.

原文English
主出版物標題In Situ and On-Site Bioremediation-2009
主出版物子標題Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium
出版狀態Published - 2009 11月 27
事件10th International In Situ and On-Site Bioremediation Symposium, In Situ and On-Site Bioremediation-2009 - Baltimore, MD, United States
持續時間: 2009 5月 52009 5月 8

出版系列

名字In Situ and On-Site Bioremediation-2009: Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium

Other

Other10th International In Situ and On-Site Bioremediation Symposium, In Situ and On-Site Bioremediation-2009
國家/地區United States
城市Baltimore, MD
期間09-05-0509-05-08

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 管理、監督、政策法律
  • 廢物管理和處置

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