Bioremediation potential of soil contaminated with highly substituted polychlorinated dibenzo-p-dioxins and dibenzofurans: Microcosm study and microbial community analysis

Wei Yu Chen, Jer Horng Wu, Yong Yu Lin, Hung Jun Huang, Juu En Chang

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

Highly chlorinated dibenzo-. p-dioxins/dibenzofurans (DD/Fs) are main hazardous dioxins, and ubiquitously distributed in the environment. To study the feasibility of bioremediation for remedying contamination of highly chlorinated dioxins, closed microcosms were constructed with soil from a chronological site under oxygen-stimulated conditions. The results showed that high levels of near-fully and fully chlorinated DD/Fs, particularly octachlorodibenzofuran were effectually reduced without accumulation of less substituted congeners. The clone library analysis of PCR-amplified 16S rRNA gene from the octachlorodibenzofuran-degrading consortia showed that 98.3% of the detected sequences were affiliated with Proteobacteria. The obtained strains with putative aromatic dioxygenase genes and abilities to repetitively grow in octachlorodibenzofuran-containing agars were closely related to members within Actinobacteria, Firmicutes, and Proteobacteria. Among them, certain Rhodococcus, Micrococcus, Mesorhizobium and Bacillus isolates could degrade octachlorodibenzofuran with efficiencies of 26-43% within 21 days. Hierarchical oligonucleotide primer extension analysis further showed that Micrococcus, Rhizobium, Pseudoxanthomonas, and Brevudimonas populations increased largely when high concentrations of octachlorodibenzofuran were reduced. Overall, our results suggest that a distinctive microbial composition and population dynamic could be required for the enhanced degradation of highly chlorinated DD/Fs in the batch microcosm and highlight a potential of bioremediation technologies in remedying polychlorinated dioxins in the polluted sites.

原文English
頁(從 - 到)351-361
頁數11
期刊Journal of Hazardous Materials
261
DOIs
出版狀態Published - 2013 10月 5

All Science Journal Classification (ASJC) codes

  • 環境工程
  • 環境化學
  • 廢物管理和處置
  • 污染
  • 健康、毒理學和誘變

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