Block of L-Type Ca2+ current by beauvericin, a toxic cyclopeptide, in the NG108-15 neuronal cell line

Sheng-Nan Wu, Hsinyo Chen, Yen-Chin Liu, Hung Ting Chiang

研究成果: Article

30 引文 (Scopus)

摘要

The effects of beauverficin, a cyclodepsipeptide compound, on ion currents in a mouse neuroblastoma and rat glioma hybrid cell line, NG108-15, were investigated with the aid of the whole-cell voltage-clamp technique. Beauvericin (0.3-100 μM) reversibly produced an inhibition of L-type voltage-dependent Ca2+ current (ICa,L) in a concentration-dependent manner. Beauvericin caused no change in the overall shape of the current-voltage relationship of ICa,L. The IC50 value of beauvericin-induced inhibition of ICa,L was 4 μM. Neither gabapentin (30 μM) nor ω-conotoxin GVIA (3 μM) had effects on ICa,L. Beauvericin (30 μM) shifted the steadystate inactivation curve of ICa,L to more negative membrane potentials by approximately - 15 mV. The inhibitory effects of beauvericin on ICa,L exhibited tonic and use-dependent characteristics. Beauvericin also suppressed ICa,L evoked by repetitive action potential waveforms effectively. However, beauvericin (30 μM) had no effect on delayed rectifier K+ current in NG105-18 cells. Under current-clamp configuration, beauvericin reduced the firing frequency of action potentials. Therefore, this study indicates that beauvericin is a relatively specific inhibitor of L-type Ca2+ current in NG108-15 cells.

原文English
頁(從 - 到)854-860
頁數7
期刊Chemical Research in Toxicology
15
發行號6
DOIs
出版狀態Published - 2002 七月 2

指紋

Cyclic Peptides
Poisons
Cells
Cell Line
Clamping devices
Action Potentials
Electric potential
Conotoxins
Depsipeptides
beauvericin
Hybrid Cells
Patch-Clamp Techniques
Neuroblastoma
Glioma
Membrane Potentials
Inhibitory Concentration 50
Rats
Ions
Membranes

All Science Journal Classification (ASJC) codes

  • Toxicology

引用此文

@article{636e7f7383f44165a91dc4deb8117c9c,
title = "Block of L-Type Ca2+ current by beauvericin, a toxic cyclopeptide, in the NG108-15 neuronal cell line",
abstract = "The effects of beauverficin, a cyclodepsipeptide compound, on ion currents in a mouse neuroblastoma and rat glioma hybrid cell line, NG108-15, were investigated with the aid of the whole-cell voltage-clamp technique. Beauvericin (0.3-100 μM) reversibly produced an inhibition of L-type voltage-dependent Ca2+ current (ICa,L) in a concentration-dependent manner. Beauvericin caused no change in the overall shape of the current-voltage relationship of ICa,L. The IC50 value of beauvericin-induced inhibition of ICa,L was 4 μM. Neither gabapentin (30 μM) nor ω-conotoxin GVIA (3 μM) had effects on ICa,L. Beauvericin (30 μM) shifted the steadystate inactivation curve of ICa,L to more negative membrane potentials by approximately - 15 mV. The inhibitory effects of beauvericin on ICa,L exhibited tonic and use-dependent characteristics. Beauvericin also suppressed ICa,L evoked by repetitive action potential waveforms effectively. However, beauvericin (30 μM) had no effect on delayed rectifier K+ current in NG105-18 cells. Under current-clamp configuration, beauvericin reduced the firing frequency of action potentials. Therefore, this study indicates that beauvericin is a relatively specific inhibitor of L-type Ca2+ current in NG108-15 cells.",
author = "Sheng-Nan Wu and Hsinyo Chen and Yen-Chin Liu and Chiang, {Hung Ting}",
year = "2002",
month = "7",
day = "2",
doi = "10.1021/tx020003k",
language = "English",
volume = "15",
pages = "854--860",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Block of L-Type Ca2+ current by beauvericin, a toxic cyclopeptide, in the NG108-15 neuronal cell line

AU - Wu, Sheng-Nan

AU - Chen, Hsinyo

AU - Liu, Yen-Chin

AU - Chiang, Hung Ting

PY - 2002/7/2

Y1 - 2002/7/2

N2 - The effects of beauverficin, a cyclodepsipeptide compound, on ion currents in a mouse neuroblastoma and rat glioma hybrid cell line, NG108-15, were investigated with the aid of the whole-cell voltage-clamp technique. Beauvericin (0.3-100 μM) reversibly produced an inhibition of L-type voltage-dependent Ca2+ current (ICa,L) in a concentration-dependent manner. Beauvericin caused no change in the overall shape of the current-voltage relationship of ICa,L. The IC50 value of beauvericin-induced inhibition of ICa,L was 4 μM. Neither gabapentin (30 μM) nor ω-conotoxin GVIA (3 μM) had effects on ICa,L. Beauvericin (30 μM) shifted the steadystate inactivation curve of ICa,L to more negative membrane potentials by approximately - 15 mV. The inhibitory effects of beauvericin on ICa,L exhibited tonic and use-dependent characteristics. Beauvericin also suppressed ICa,L evoked by repetitive action potential waveforms effectively. However, beauvericin (30 μM) had no effect on delayed rectifier K+ current in NG105-18 cells. Under current-clamp configuration, beauvericin reduced the firing frequency of action potentials. Therefore, this study indicates that beauvericin is a relatively specific inhibitor of L-type Ca2+ current in NG108-15 cells.

AB - The effects of beauverficin, a cyclodepsipeptide compound, on ion currents in a mouse neuroblastoma and rat glioma hybrid cell line, NG108-15, were investigated with the aid of the whole-cell voltage-clamp technique. Beauvericin (0.3-100 μM) reversibly produced an inhibition of L-type voltage-dependent Ca2+ current (ICa,L) in a concentration-dependent manner. Beauvericin caused no change in the overall shape of the current-voltage relationship of ICa,L. The IC50 value of beauvericin-induced inhibition of ICa,L was 4 μM. Neither gabapentin (30 μM) nor ω-conotoxin GVIA (3 μM) had effects on ICa,L. Beauvericin (30 μM) shifted the steadystate inactivation curve of ICa,L to more negative membrane potentials by approximately - 15 mV. The inhibitory effects of beauvericin on ICa,L exhibited tonic and use-dependent characteristics. Beauvericin also suppressed ICa,L evoked by repetitive action potential waveforms effectively. However, beauvericin (30 μM) had no effect on delayed rectifier K+ current in NG105-18 cells. Under current-clamp configuration, beauvericin reduced the firing frequency of action potentials. Therefore, this study indicates that beauvericin is a relatively specific inhibitor of L-type Ca2+ current in NG108-15 cells.

UR - http://www.scopus.com/inward/record.url?scp=0035998285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035998285&partnerID=8YFLogxK

U2 - 10.1021/tx020003k

DO - 10.1021/tx020003k

M3 - Article

C2 - 12067253

AN - SCOPUS:0035998285

VL - 15

SP - 854

EP - 860

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

IS - 6

ER -