Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds

Yu Chun Wu, Shyh-Yu Shaw, Hong Ru Lin, Tzer-Min Lee, Chyun-Yu Yang

研究成果: Article同行評審

119 引文 斯高帕斯(Scopus)

摘要

Using natural materials to coat the scaffolds used for tissue-engineered bone-repair techniques is expected to increase osteoblast adhesion to the scaffold and to express normal physiological function. To test this hypothesis, we therefore modified poly(dl-lactic-co-glycolic acid) (PLGA) substrate by coating it with natural biomaterial solutions of collagen, chitosan, or N-succinyl-chitosan, and then used these three combinations as scaffolds to evaluate their effects on osteoblast attachment, proliferation, and differentiation. The results demonstrated that the pore size of scaffolds ranging from 125-500 μm did not affect the osteoblast phenotype; however, the surface modification of the scaffolds coated with these natural biomaterials did. Collagen increased cell attachment and proliferation, but chitosan and N-succinyl-chitosan decreased them. Chitosan and N-succinyl-chitosan increased differentiation, but collagen decreased it. These results provide us a new strategy for modifying microenvironments to increase osteoblast adhesion, proliferation, and differentiation on PLGA scaffolds, a strategy that might be useful for tissue regeneration.

原文English
頁(從 - 到)896-904
頁數9
期刊Biomaterials
27
發行號6
DOIs
出版狀態Published - 2006 二月 1

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 陶瓷和複合材料
  • 生物物理學
  • 生物材料
  • 材料力學

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