Butyrate and TGF-β Downregulate Na,K-ATPase Expression in Cultured Proximal Tubule Cells

研究成果: Article

14 引文 (Scopus)

摘要

Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.

原文English
頁(從 - 到)57-66
頁數10
期刊Biochemical and Biophysical Research Communications
215
發行號1
DOIs
出版狀態Published - 1995 十月 4

指紋

Butyrates
Adenosine Triphosphatases
Down-Regulation
Messenger RNA
Growth Inhibitors
Proximal Kidney Tubule
Time and motion study
sodium-translocating ATPase
Gene expression
Anti-Idiotypic Antibodies
Proteins
Gene Expression
Antibodies
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

引用此文

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abstract = "Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.",
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AU - Tang, M. J.

AU - Wang, Y. K.

AU - Lin, H. H.

PY - 1995/10/4

Y1 - 1995/10/4

N2 - Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.

AB - Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.

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