摘要
Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.
| 原文 | English |
|---|---|
| 頁（從 - 到） | 57-66 |
| 頁數 | 10 |
| 期刊 | Biochemical and Biophysical Research Communications |
| 卷 | 215 |
| 發行號 | 1 |
| DOIs | |
| 出版狀態 | Published - 1995 十月 4 |
指紋
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
引用此文
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Butyrate and TGF-β Downregulate Na,K-ATPase Expression in Cultured Proximal Tubule Cells. / Tang, M. J.; Wang, Y. K.; Lin, H. H.
於: Biochemical and Biophysical Research Communications, 卷 215, 編號 1, 04.10.1995, p. 57-66.研究成果: Article
TY - JOUR
T1 - Butyrate and TGF-β Downregulate Na,K-ATPase Expression in Cultured Proximal Tubule Cells
AU - Tang, M. J.
AU - Wang, Y. K.
AU - Lin, H. H.
PY - 1995/10/4
Y1 - 1995/10/4
N2 - Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.
AB - Attempts were made to examine the effect of growth inhibitors on regulation of Na,K-ATPase in primary cultures of renal proximal tubule cells. We observed that both TGF-β and butyrate induced dose dependent decrease of Na,K-ATPase activity. The time course studies showed that the effect of TGF-β preceded the effect of butyrate on inhibition of Na,K-ATPase activity. Both butyrate- and TGF-β-induced inhibition of this enzyme were in general mediated by transcriptional decreases of αand β mRNA and therefore also protein abundance. Like its effect on Na,K-ATPase activity, TGF-β compared to butyrate induced much earlier decline in Na,K-ATPase α and β mRNA and protein abundance. Butyrate did not affect gene expression of TGF-β and anti-TGF-β antibody had no effect on butyrate-induced inhibition of DNA synthesis. Obviously, both butyrate and TGF-β can inhibit the expression of Na,K-ATPase α and β mRNA, but TGF-β does not mediate butyrate′s effect.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028970516&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.2433
DO - 10.1006/bbrc.1995.2433
M3 - Article
C2 - 7575625
AN - SCOPUS:0028970516
VL - 215
SP - 57
EP - 66
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -