Butyrate regulates the expression of c-Src and focal adhesion kinase and inhibits cell invasion of human colon cancer cells

Jenq Chang Lee, Ming Chei Maa, Hsiu Shan Yu, Jung Hui Wang, Chia Kuang Yen, Shan Tair Wang, Yen Jen Chen, Yuan Liu, Ying Tai Jin, Tzeng Horng Leu

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26 引文 斯高帕斯(Scopus)

摘要

Epidemiological studies indicate that dietary fiber-derived fermentation products such as butyrate can prevent colon cancer development. To further dissect the roie of butyrate in anticarcinogenesis, its effect on cellular growth and invasion as well as the expression of c-Src and FAK, two mutually interactive nonreceptor tyrosine kinases, in three different human colon cancer cell lines (Caco-2, SW480, and SW620) were investigated. In addition to growth inhibition, butyrate treatment results in a significant downregulation of c-Src and FAK in human colon cancer cells, which can be attributable to their reduced transcripts and implicates the participation of a butyrate-sensitive pathway in modulating their expression. Concurrent to butyrate-reduced c-Src and FAK expression is the decrease of FAK Tyr-397 phosphorylation. Besides, butyrate also abolished the secretion of MMP-2 and MMP-9. And these butyrate-mediated effects severely impaired invasion of SW620 cells through Matrigel in vitro. Interestingly, in situ parallel enhancement of c-Src and FAK was also observed in human colorectal tumor specimens. These results imply that by virtue of suppression of c-Src and FAK along with other butyrate targets in colonocytes, butyrate could effectively inhibit tumor growth and invasion.

原文English
頁(從 - 到)207-214
頁數8
期刊Molecular Carcinogenesis
43
發行號4
DOIs
出版狀態Published - 2005 八月

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 癌症研究

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