Calcium phosphate particles stimulate exosome secretion from phagocytes for the enhancement of drug delivery

Yan Jye Shyong, Kuo Chi Chang, Feng Huei Lin

研究成果: Article

摘要

Exosomes are attractive potential carriers for drug delivery because of their natural function of transferring biomolecules among cells without eliciting immune responses. However, an obstacle to the application of exosomes for drug delivery is the difficulty in collecting sufficient numbers of these vesicles. In this study, we demonstrate treatment with calcium phosphate (CaP) particles could increase over two-fold the number of exosomes secreted from macrophage-like RAW264.7 cells and monocyte-like THP-1 cells. CaP particles were easily internalized into cells and dissolved in acidic late-endosomes or lysosomes, resulting in the rupture of their membranes followed by the release of Ca2+ into cytosol. Moreover, we found that exosomes secreted from cells treated with CaP particles are not contaminated by the Ca2+ released from CaP; the Ca2+ contents in exosomes secreted from CaP particle-treated cells were similar to that in exosomes from untreated control cells. This study highlights the potential for the efficient production of exosomes using CaP particles for drug delivery.

原文English
頁(從 - 到)391-397
頁數7
期刊Colloids and Surfaces B: Biointerfaces
171
DOIs
出版狀態Published - 2018 十一月 1

指紋

Exosomes
secretions
calcium phosphates
Calcium phosphate
Phagocytes
Drug delivery
delivery
drugs
augmentation
cells
Pharmaceutical Preparations
monocytes
lysosomes
Macrophages
Biomolecules
Drug Carriers
macrophages
Endosomes
Lysosomes
calcium phosphate

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

引用此文

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abstract = "Exosomes are attractive potential carriers for drug delivery because of their natural function of transferring biomolecules among cells without eliciting immune responses. However, an obstacle to the application of exosomes for drug delivery is the difficulty in collecting sufficient numbers of these vesicles. In this study, we demonstrate treatment with calcium phosphate (CaP) particles could increase over two-fold the number of exosomes secreted from macrophage-like RAW264.7 cells and monocyte-like THP-1 cells. CaP particles were easily internalized into cells and dissolved in acidic late-endosomes or lysosomes, resulting in the rupture of their membranes followed by the release of Ca2+ into cytosol. Moreover, we found that exosomes secreted from cells treated with CaP particles are not contaminated by the Ca2+ released from CaP; the Ca2+ contents in exosomes secreted from CaP particle-treated cells were similar to that in exosomes from untreated control cells. This study highlights the potential for the efficient production of exosomes using CaP particles for drug delivery.",
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