Calibration of a lactic-acid model for simulating biofilm-induced degradation of the dentin-composite interface

Laikuan Zhu, Yuping Li, Carola A. Carrera, Yung-Chung Chen, Mingyu Li, Alex Fok

研究成果: Article

3 引文 (Scopus)

摘要

Objective To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. Methods Dentin-composite disks (5-mm dia. × 2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4.5 for up to 48 h. Diametral compression was performed to measure the reduction in bond strength of the dentin-composite disks following acid challenge. Scanning electron microscopy (SEM) was used to examine decalcification of dentin and fracture modes of the disks. To better understand the degradation process, micro-computed tomography, in combination with a radiopaque dye (AgNO3), was used to assess interfacial leakage in 3D longitudinally, while SEM was used to determine the path of leakage. One-way analysis of variance (ANOVA) was used to analyze the results, with the level of statistical significance set at p < 0.05. The results were compared with those obtained previously using multi-species biofilms for verification and calibration purposes. Results After 48 h of acid challenge, the debonding load of both the LS- and Z100-filled disks reduced significantly (p < 0.05). In the Z100-filled disks, debonding mostly occurred at the adhesive-dentin interface, while in the LS-filled disks, this happened at the adhesive-composite interface, instead. The degree of dentin demineralization, the reduction in debonding load and the modes of failure observed were very similar to those induced by multi-species oral biofilms found in the previous work. Leakage of AgNO3 occurred mainly along the hybrid layer. The specimens filled with Z100 had a thicker hybrid layer (∼6.5 μm), which exhibited more interfacial leakage than those filled with LS. Significance The chemical model with lactic acid used in this study can induce degradation to the dentin-composite interface similar to those produced by multi-species biofilms. With appropriate calibration, this could provide an effective in vitro method for ageing composite restorations in assessing their potential clinical performance.

原文English
頁(從 - 到)1315-1323
頁數9
期刊Dental Materials
33
發行號11
DOIs
出版狀態Published - 2017 十一月 1

指紋

Biofilms
Dentin
Lactic acid
Calibration
Lactic Acid
Degradation
Composite materials
Debonding
Chemical Models
Adhesives
Electron Scanning Microscopy
Varnish
Nails
Scanning electron microscopy
Acids
Paint
Incisor
Analysis of variance (ANOVA)
Restoration
Tomography

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

引用此文

Zhu, Laikuan ; Li, Yuping ; Carrera, Carola A. ; Chen, Yung-Chung ; Li, Mingyu ; Fok, Alex. / Calibration of a lactic-acid model for simulating biofilm-induced degradation of the dentin-composite interface. 於: Dental Materials. 2017 ; 卷 33, 編號 11. 頁 1315-1323.
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abstract = "Objective To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. Methods Dentin-composite disks (5-mm dia. × 2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4.5 for up to 48 h. Diametral compression was performed to measure the reduction in bond strength of the dentin-composite disks following acid challenge. Scanning electron microscopy (SEM) was used to examine decalcification of dentin and fracture modes of the disks. To better understand the degradation process, micro-computed tomography, in combination with a radiopaque dye (AgNO3), was used to assess interfacial leakage in 3D longitudinally, while SEM was used to determine the path of leakage. One-way analysis of variance (ANOVA) was used to analyze the results, with the level of statistical significance set at p < 0.05. The results were compared with those obtained previously using multi-species biofilms for verification and calibration purposes. Results After 48 h of acid challenge, the debonding load of both the LS- and Z100-filled disks reduced significantly (p < 0.05). In the Z100-filled disks, debonding mostly occurred at the adhesive-dentin interface, while in the LS-filled disks, this happened at the adhesive-composite interface, instead. The degree of dentin demineralization, the reduction in debonding load and the modes of failure observed were very similar to those induced by multi-species oral biofilms found in the previous work. Leakage of AgNO3 occurred mainly along the hybrid layer. The specimens filled with Z100 had a thicker hybrid layer (∼6.5 μm), which exhibited more interfacial leakage than those filled with LS. Significance The chemical model with lactic acid used in this study can induce degradation to the dentin-composite interface similar to those produced by multi-species biofilms. With appropriate calibration, this could provide an effective in vitro method for ageing composite restorations in assessing their potential clinical performance.",
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Calibration of a lactic-acid model for simulating biofilm-induced degradation of the dentin-composite interface. / Zhu, Laikuan; Li, Yuping; Carrera, Carola A.; Chen, Yung-Chung; Li, Mingyu; Fok, Alex.

於: Dental Materials, 卷 33, 編號 11, 01.11.2017, p. 1315-1323.

研究成果: Article

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AU - Li, Mingyu

AU - Fok, Alex

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N2 - Objective To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. Methods Dentin-composite disks (5-mm dia. × 2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4.5 for up to 48 h. Diametral compression was performed to measure the reduction in bond strength of the dentin-composite disks following acid challenge. Scanning electron microscopy (SEM) was used to examine decalcification of dentin and fracture modes of the disks. To better understand the degradation process, micro-computed tomography, in combination with a radiopaque dye (AgNO3), was used to assess interfacial leakage in 3D longitudinally, while SEM was used to determine the path of leakage. One-way analysis of variance (ANOVA) was used to analyze the results, with the level of statistical significance set at p < 0.05. The results were compared with those obtained previously using multi-species biofilms for verification and calibration purposes. Results After 48 h of acid challenge, the debonding load of both the LS- and Z100-filled disks reduced significantly (p < 0.05). In the Z100-filled disks, debonding mostly occurred at the adhesive-dentin interface, while in the LS-filled disks, this happened at the adhesive-composite interface, instead. The degree of dentin demineralization, the reduction in debonding load and the modes of failure observed were very similar to those induced by multi-species oral biofilms found in the previous work. Leakage of AgNO3 occurred mainly along the hybrid layer. The specimens filled with Z100 had a thicker hybrid layer (∼6.5 μm), which exhibited more interfacial leakage than those filled with LS. Significance The chemical model with lactic acid used in this study can induce degradation to the dentin-composite interface similar to those produced by multi-species biofilms. With appropriate calibration, this could provide an effective in vitro method for ageing composite restorations in assessing their potential clinical performance.

AB - Objective To verify and calibrate a chemical model for simulating the degradation of the dentin-composite interface induced by multi-species oral biofilms in vitro. Methods Dentin-composite disks (5-mm dia. × 2-mm thick) were made from bovine incisor roots and filled with either Z100™ (Z100) or Filtek™ LS (LS) composite. The disks, which were covered with nail varnish, but with one of the dentin-composite margins exposed, were immersed in lactic acid solution at pH 4.5 for up to 48 h. Diametral compression was performed to measure the reduction in bond strength of the dentin-composite disks following acid challenge. Scanning electron microscopy (SEM) was used to examine decalcification of dentin and fracture modes of the disks. To better understand the degradation process, micro-computed tomography, in combination with a radiopaque dye (AgNO3), was used to assess interfacial leakage in 3D longitudinally, while SEM was used to determine the path of leakage. One-way analysis of variance (ANOVA) was used to analyze the results, with the level of statistical significance set at p < 0.05. The results were compared with those obtained previously using multi-species biofilms for verification and calibration purposes. Results After 48 h of acid challenge, the debonding load of both the LS- and Z100-filled disks reduced significantly (p < 0.05). In the Z100-filled disks, debonding mostly occurred at the adhesive-dentin interface, while in the LS-filled disks, this happened at the adhesive-composite interface, instead. The degree of dentin demineralization, the reduction in debonding load and the modes of failure observed were very similar to those induced by multi-species oral biofilms found in the previous work. Leakage of AgNO3 occurred mainly along the hybrid layer. The specimens filled with Z100 had a thicker hybrid layer (∼6.5 μm), which exhibited more interfacial leakage than those filled with LS. Significance The chemical model with lactic acid used in this study can induce degradation to the dentin-composite interface similar to those produced by multi-species biofilms. With appropriate calibration, this could provide an effective in vitro method for ageing composite restorations in assessing their potential clinical performance.

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