Ovarian cancer has been one of the leading causes of death for women. However, it is still very challenging for its early diagnosis. The tedious conventional methods for screening specific biomarkers need at least two weeks or more. It was reported that cancer-specific oligopeptides could be screened from a phage display library. In this study, a new integrated microfluidic chip was developed to perform the entire screening process for specific oligopeptide selection of ovarian cancer cells. The screened oligopeptide was demonstrated to have high affinity with ovarian cancer cells and relatively low capture rate with other types of cancer cells. It is therefore promising to use the screened oligopeptide for isolation of ovarian cancer cells in the near future.