Ca2+-regulated cell migration revealed by optogenetically engineered Ca2+ oscillations

Yi Shyun Lai, Ya Han Chang, Yong Yi Chen, Jixuan Xu, Chi Sian Yu, Su Jing Chang, Pai Sheng Chen, Shaw Jenq Tsai, Wen Tai Chiu

研究成果: Article同行評審

摘要

The ability of a single Ca2+ ion to play an important role in cell biology is highlighted by the need for cells to form Ca2+ signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca2+ concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca2+ translocating channelrhodopsin (CatCh) were used to mediate Ca2+ influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca2+-dependent transcription factors and certain kinases can be activated by specific Ca2+ waves. Using a wound-healing assay, we found that low-frequency Ca2+ oscillations increased cell migration through the activation of NF-κB. This study explores the regulation of cell migration by Ca2+ signals. Thus, we can choose optical parameters to modulate Ca2+ waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell-signaling mechanisms in the future.

原文English
頁(從 - 到)4681-4693
頁數13
期刊Journal of Cellular Physiology
236
發行號6
DOIs
出版狀態Accepted/In press - 2020

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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