Cell Cycle-Dependent Localization of Dynactin Subunit p150glued at Centrosome

Ting Yu Chen, Jhih Siang Syu, Tsung Yu Han, Hui Ling Cheng, Fu-I Lu, Chia-Yih Wang

研究成果: Article

9 引文 (Scopus)

摘要

p150glued is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150glued also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150glued correlated with cell cycle progression. Here we found that p150glued was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150glued became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150glued was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150glued appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150glued did not induce centrosome amplification or splitting but inhibited U2OS cell growth. G1 arrest and reduced EdU incorporation were observed in p150glued deficient U2OS cells. In addition, cyclin E was downregulated following p150glued depletion. The p53/p21 signaling was activated indicating that CDKs were inactivated. The reduced cell growth was ameliorated in the p150glued depleted cells when treated with p53 inhibitor. Thus, we have identified the centrosomal targeting of p150glued in distinct cell cycle stage and uncovered its role in controlling G1/S transition. J. Cell. Biochem. 116: 2049-2060, 2015.

原文English
頁(從 - 到)2049-2060
頁數12
期刊Journal of Cellular Biochemistry
116
發行號9
DOIs
出版狀態Published - 2015 九月 1

指紋

Centrosome
Cell Cycle
Cells
Cell growth
Centrioles
Cilia
Dyneins
Cyclin E
Internship and Residency
Starvation
Phosphorylation
Microtubules
Amplification
Spindle Poles
Poles
Proteins
Spindle Apparatus
G2 Phase
Protein Subunits
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

引用此文

Chen, Ting Yu ; Syu, Jhih Siang ; Han, Tsung Yu ; Cheng, Hui Ling ; Lu, Fu-I ; Wang, Chia-Yih. / Cell Cycle-Dependent Localization of Dynactin Subunit p150glued at Centrosome. 於: Journal of Cellular Biochemistry. 2015 ; 卷 116, 編號 9. 頁 2049-2060.
@article{a1c1ac8a85054a56b83d52f9221eb760,
title = "Cell Cycle-Dependent Localization of Dynactin Subunit p150glued at Centrosome",
abstract = "p150glued is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150glued also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150glued correlated with cell cycle progression. Here we found that p150glued was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150glued became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150glued was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150glued appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150glued did not induce centrosome amplification or splitting but inhibited U2OS cell growth. G1 arrest and reduced EdU incorporation were observed in p150glued deficient U2OS cells. In addition, cyclin E was downregulated following p150glued depletion. The p53/p21 signaling was activated indicating that CDKs were inactivated. The reduced cell growth was ameliorated in the p150glued depleted cells when treated with p53 inhibitor. Thus, we have identified the centrosomal targeting of p150glued in distinct cell cycle stage and uncovered its role in controlling G1/S transition. J. Cell. Biochem. 116: 2049-2060, 2015.",
author = "Chen, {Ting Yu} and Syu, {Jhih Siang} and Han, {Tsung Yu} and Cheng, {Hui Ling} and Fu-I Lu and Chia-Yih Wang",
year = "2015",
month = "9",
day = "1",
doi = "10.1002/jcb.25160",
language = "English",
volume = "116",
pages = "2049--2060",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "9",

}

Cell Cycle-Dependent Localization of Dynactin Subunit p150glued at Centrosome. / Chen, Ting Yu; Syu, Jhih Siang; Han, Tsung Yu; Cheng, Hui Ling; Lu, Fu-I; Wang, Chia-Yih.

於: Journal of Cellular Biochemistry, 卷 116, 編號 9, 01.09.2015, p. 2049-2060.

研究成果: Article

TY - JOUR

T1 - Cell Cycle-Dependent Localization of Dynactin Subunit p150glued at Centrosome

AU - Chen, Ting Yu

AU - Syu, Jhih Siang

AU - Han, Tsung Yu

AU - Cheng, Hui Ling

AU - Lu, Fu-I

AU - Wang, Chia-Yih

PY - 2015/9/1

Y1 - 2015/9/1

N2 - p150glued is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150glued also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150glued correlated with cell cycle progression. Here we found that p150glued was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150glued became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150glued was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150glued appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150glued did not induce centrosome amplification or splitting but inhibited U2OS cell growth. G1 arrest and reduced EdU incorporation were observed in p150glued deficient U2OS cells. In addition, cyclin E was downregulated following p150glued depletion. The p53/p21 signaling was activated indicating that CDKs were inactivated. The reduced cell growth was ameliorated in the p150glued depleted cells when treated with p53 inhibitor. Thus, we have identified the centrosomal targeting of p150glued in distinct cell cycle stage and uncovered its role in controlling G1/S transition. J. Cell. Biochem. 116: 2049-2060, 2015.

AB - p150glued is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150glued also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150glued correlated with cell cycle progression. Here we found that p150glued was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150glued became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150glued was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150glued appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150glued did not induce centrosome amplification or splitting but inhibited U2OS cell growth. G1 arrest and reduced EdU incorporation were observed in p150glued deficient U2OS cells. In addition, cyclin E was downregulated following p150glued depletion. The p53/p21 signaling was activated indicating that CDKs were inactivated. The reduced cell growth was ameliorated in the p150glued depleted cells when treated with p53 inhibitor. Thus, we have identified the centrosomal targeting of p150glued in distinct cell cycle stage and uncovered its role in controlling G1/S transition. J. Cell. Biochem. 116: 2049-2060, 2015.

UR - http://www.scopus.com/inward/record.url?scp=84937460079&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937460079&partnerID=8YFLogxK

U2 - 10.1002/jcb.25160

DO - 10.1002/jcb.25160

M3 - Article

C2 - 25774020

AN - SCOPUS:84937460079

VL - 116

SP - 2049

EP - 2060

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 9

ER -