Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome

Masayuki Hashimoto, Toshiharu Ichimura, Hiroshi Mizoguchi, Kimie Tanaka, Kazuyuki Fujimitsu, Kenji Keyamura, Tomotake Ote, Takehiro Yamakawa, Yukiko Yamazaki, Hideo Mori, Tsutomu Katayama, Jun Ichi Kato

研究成果: Article同行評審

186 引文 斯高帕斯(Scopus)

摘要

The minimization of a genome is necessary to identify experimentally the minimal gene set that contains only those genes that are essential and sufficient to sustain a functioning cell. Recent developments in genetic techniques have made it possible to generate bacteria with a markedly reduced genome. We developed a simple system for formation of markerless chromosomal deletions, and constructed and characterized a series of large-scale chromosomal deletion mutants of Escherichia coli that lack between 2.4 and 29.7% of the parental chromosome. Combining deletion mutations changes cell length and width, and the mutant cells with larger deletions were even longer and wider than the parental cells. The nucleoid organization of the mutants is also changed: the nucleoids occur as multiple small nucleoids and are localized peripherally near the envelope. Inhibition of translation causes them to condense into one or two packed nucleoids, suggesting that the coupling of transcription and translation of membrane proteins peripherally localizes chromosomes. Because these phenotypes are similar to those of spherical cells, those may be a consequence of the morphological change. Based on the nucleoid localization observed with these mutants, we discuss the cellular nucleoid dynamics.

原文English
頁(從 - 到)137-149
頁數13
期刊Molecular Microbiology
55
發行號1
DOIs
出版狀態Published - 2005 一月

All Science Journal Classification (ASJC) codes

  • 微生物學
  • 分子生物學

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