Cell transplantation for myocardial injury: A preliminary comparative study

Chung Dann Kan, Hsin Ling Lee, Yu Jen Yang

研究成果: Article同行評審

7 引文 斯高帕斯(Scopus)

摘要

Background aims. Cell transplantation may restore viable muscle after myocardial infarction. Because many studies have focused on one cell type, we compared the characteristics of skeletal myoblasts (SKM), bone marrow stromal/stem cells (BMSC) and smooth muscle cells (SMC) and their effects on cardiac function after myocardial injury. Methods. In vitro cell characteristics, including proliferation, hypoxic survival and vascular endothelial cell growth factor (VEGF) expression, of SKM, BMSC and SMC were compared. An in vivo left anterior descending artery ligation rat model was used, and cells were implanted into the infarct (n = 16 per cell type). Cell survival was determined by PKH26 staining and real-time polymerase chain reaction (PCR). Cardiac function, tissue VEGF and stem cell factor (SCF) expression and vasculogenesis were evaluated. Results. Although cell morphologies were distinct, in vitro proliferation was similar. In vitro studies showed that SKM had the highest hypoxic survival, whereas BMSC had the lowest hypoxic survival but the highest VEGF expression. After implantation, SKM showed the highest overall survival and in vivo SCF expression, and both SMC and SKM expressed the highest VEGF levels. Vasculogenesis was significantly (P < 0.001) improved after transplantation of each cell type. Overall, BMSC and SKM promoted the greatest improvement in cardiac function. Conclusions. SKM, BMSC and SMC expressed VEGF and SCF and promoted vasculogenesis. Although BMSC showed the greatest regenerative potential relative to cell survival and growth factor expression, the greatest improvement in cardiac function was observed with BMSC and SKM.

原文English
頁(從 - 到)692-700
頁數9
期刊Cytotherapy
12
發行號5
DOIs
出版狀態Published - 2010 九月

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 免疫學
  • 腫瘤科
  • 遺傳學(臨床)
  • 細胞生物學
  • 癌症研究
  • 移植

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