TY - JOUR
T1 - Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E
AU - Ho, Shih Hsin
AU - Huang, Shu Wen
AU - Chen, Chun Yen
AU - Hasunuma, Tomohisa
AU - Kondo, Akihiko
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge the financial support fromtheCore Research for Evolutional Science and Technology (CREST) of the Promoting Globalization on Strategic Basic Research Programs of the Japan Science and Technology Agency (JST). The support by the top university project of NCKU and by Taiwan’s National Science Council under grant numbers NSC 101-3113-P-110-002 , NSC 101-3113-E-006-015 , and NSC 101-3113-E- 006-016 .
PY - 2013/5
Y1 - 2013/5
N2 - In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631gL-1d-1, respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.
AB - In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631gL-1d-1, respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.
UR - http://www.scopus.com/inward/record.url?scp=84876477566&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876477566&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.10.100
DO - 10.1016/j.biortech.2012.10.100
M3 - Article
C2 - 23186680
AN - SCOPUS:84876477566
SN - 0960-8524
VL - 135
SP - 157
EP - 165
JO - Bioresource technology
JF - Bioresource technology
ER -
