Characterization of monoclonal antibodies to chitinase A1 and enhancement of chitinase A1 activity by monoclonal antibodies

Sakari Sekine, Yashushi Ito, Masayuki Hashimoto, Hirosato Tanaka, Takeshi Watanabe

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

A total of eleven hybridomas which secrete antibodies against chitinase A1 were established. Among the eleven monoclonal antibodies (MAbs) obtained, six recognized the catalytic domain, three recognized the type III region and two recognized the chitin binding domain of chitinase A1. Of the two MAbs which recognized the chitin binding domain one was found to also react with chitinase D, but none of the other MAbs which recognized either the type III region or the chitin binding domain reacted with chitinase D despite the extensive amino acid sequence similarity between both the type III and chitin binding domains of the two chitinases. Two of the eleven MAbs enhanced chitinase activity significantly, while the other MAbs did not have any significant effect on chitinase A1 activity.

原文English
頁(從 - 到)7-16
頁數10
期刊Biochemical and Biophysical Research Communications
204
發行號1
DOIs
出版狀態Published - 1994 十月 15

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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