Characterization of oral squamous cell carcinoma based on higher-harmonic generation microscopy

Ming Rung Tsai, Dar Bin Shieh, Pei Jen Lou, Chih Feng Lin, Chi Kuang Sun

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)


In vivo higher-harmonic generation microscopy (HGM) performed on healthy human oral mucosa not only provides images with a <500 nm lateral resolution at a 280 μm penetration depth, but also leaves no photodamages in the tissues. These advantages suggest that HGM could serve as an ideal virtual biopsy tool for in vivo, in situ, and immediate histopathological diagnosis of oral cancer. However, translation of such mechanism for clinical cancer diagnosis requires evidence based algorithm capable to differentiate cancerous tissues from normal. It is thus critical to investigate if the endogenous contrast provided by the HGM would be high enough to differentiate cancerous versus normal tissues in human oral mucosa. In this report, ex vivo HGM study was performed on the cancerous mucosa from 10 patients with oral squamous cell carcinoma. Compared with histology, HGM revealed histopathological features including the cytological abnormalities, loss of differentiation, interruption of basement membrane, and irregular epithelial stratification in all 10 specimens. In addition, distinct patterns of collagen fibers and increased distribution area of actin filaments in tumor cells were noted. These results indicate HGM holds great potential for the optical biopsy screening of oral cancer lesions.

頁(從 - 到)415-424
期刊Journal of Biophotonics
出版狀態Published - 2012 5月

All Science Journal Classification (ASJC) codes

  • 一般化學
  • 一般材料科學
  • 一般生物化學,遺傳學和分子生物學
  • 一般工程
  • 一般物理與天文學


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