Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood

Pin Chuan Chen, Chih Chun Chen, Kung Chia Young

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

In this study, we developed a fully thermoplastic microfiltration chip for the separation of blood plasma from human blood. Spiral microchannels were manufactured on a PMMA substrate using a micromilling machine, and a commercial polycarbonate membrane was bonded between two thermoplastic substrates. To achieve an excellent bonding between the commercial membrane and the thermoplastic substrates, we used a two-step injection and curing procedure of UV adhesive into a ring-shaped structure around the microchannel to efficiently prevent leakage during blood filtration. We performed multiple filtration experiments using human blood to compare the influence of three factors on separation efficiency: hematocrit level (40%, 23.2%, and 10.9%), membrane pore size (5 lm, 2 lm, and 1 lm), and flow rate (0.02ml/min, 0.06 ml/min, 0.1ml/min). To prevent hemolysis, the pressure within the microchannel was kept below 0.5 bars throughout all filtration experiments. The experimental results clearly demonstrated the following: (1) The proposed microfiltration chip is able to separate white blood cells and red blood cells from whole human blood with a separation efficiency that exceeds 95%; (2) no leakage occurred during any of the experiments, thereby demonstrating the effectiveness of bonding a commercial membrane with a thermoplastic substrate using UV adhesive in a ring-shaped structure; (3) separation efficiency can be increased by using a membrane with smaller pore size, by using diluted blood with lower hematocrit, or by injecting blood into the microfiltration chip at a lower flow rate.

原文English
文章編號054112
期刊Biomicrofluidics
10
發行號5
DOIs
出版狀態Published - 2016 9月 1

All Science Journal Classification (ASJC) codes

  • 生物醫學工程
  • 材料科學(全部)
  • 凝聚態物理學
  • 流體流動和轉移過程
  • 膠體和表面化學

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