Charge remote fragmentation of peptides following attachment of a fixed positive charge: A matrix-assisted laser desorption/ionization postsource decay study

Pao Chi Liao, Zhi Heng Huang, John Allison

研究成果: Article同行評審

55 引文 斯高帕斯(Scopus)

摘要

A fixed positive charge can be placed at the N-terminus of a peptide by addition of a tris[(2,4,6-trimethoxyphenyl)phosphonium]acetyl group. The usefulness of these charged derivatives has been demonstrated in fast atom bombardment mass spectrometry and in matrix-assisted laser desorption/ionization mass spectrometry. After ion formation and acceleration, these derivatized peptide ions dissociate and their fragment ions can be analyzed in a postsource decay experiment by using a time-of- flight mass spectrometer. The matrix-assisted laser desorption/ionization postsource decay spectra are very different from what may be expected based on fragment ions observed from protonated peptide molecules. Cleavage of CHR- C(O) bonds dominates to form a series of a-type ions. Mechanistic possibilities are evaluated. When aspartic acid residues are encountered, the chemistry radically changes. This appears to be due to the formation of geometrically accessible intermediates that can dissociate via low energy processes. This charge-remote chemistry parallels that for much simpler systems, resulting in spectra that are very easy to interpret.

原文English
頁(從 - 到)501-509
頁數9
期刊Journal of the American Society for Mass Spectrometry
8
發行號5
DOIs
出版狀態Published - 1997 五月

All Science Journal Classification (ASJC) codes

  • 結構生物學
  • 光譜

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