TY - JOUR
T1 - Chitosan oligosaccharides suppress nuclear factor-Kappa B activation and ameliorate experimental autoimmune uveoretinitis in mice
AU - Hsu, Sheng Min
AU - Yang, Chang Hao
AU - Tsai, Hsien Yang
AU - Lin, Chia Jhen
AU - Fang, Yi Hsuan
AU - Shieh, Chi Chang
AU - Chen, Shun Hua
N1 - Funding Information:
Funding: This research was funded by National Cheng Kung University Hospital, grant number NCKUH-10507003 (to S.-M.H. and S.-H.C.).
PY - 2020/11/1
Y1 - 2020/11/1
N2 - We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of COS or phosphate-buffered saline (PBS) was given to mice daily after EAU induction. We found that mouse EAU is ameliorated by the high-dose COS treatment when compared with PBS treatment. In the retinas of high-dose COS-treated mice, the nuclear translocation of NF-κB subunit (p65) was suppressed, and the expression of several key EAU inflammatory mediators, IFN-γ, TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-10, IL-17 and MCP-1 was lowered. These results suggest that COS may be a potential treatment for posterior uveitis.
AB - We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of COS or phosphate-buffered saline (PBS) was given to mice daily after EAU induction. We found that mouse EAU is ameliorated by the high-dose COS treatment when compared with PBS treatment. In the retinas of high-dose COS-treated mice, the nuclear translocation of NF-κB subunit (p65) was suppressed, and the expression of several key EAU inflammatory mediators, IFN-γ, TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-10, IL-17 and MCP-1 was lowered. These results suggest that COS may be a potential treatment for posterior uveitis.
UR - http://www.scopus.com/inward/record.url?scp=85095590326&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85095590326&partnerID=8YFLogxK
U2 - 10.3390/ijms21218326
DO - 10.3390/ijms21218326
M3 - Article
C2 - 33171990
AN - SCOPUS:85095590326
VL - 21
SP - 1
EP - 11
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 21
M1 - 8326
ER -