Chronically monitoring of optogenetic stimulation-induced neural and hemodynamic response

Chun Wei Wu, Jia-Jin Chen

研究成果: Conference article

摘要

Understanding the link between neuronal activity and cerebral hemodynamics, known as neurovascular coupling (NVC), is critical for studying cerebral vascular dysfunctions. Optogenetics, which involves using light to control electrical activity of opsin-expressing neurons, is an approach to monitor and modulate functions of specific neurons in neuronal networks. Recent studies suggested that optogenetic stimulation induced hemodynamic response could be a good model for studying the mechanism of NVC. The aim of the study is to develop an optogenetic platform for long-term monitoring of the cerebral hemodynamics. An implantable optrode for optical stimulation and neural recording in addition to near-infrared spectroscopy (NIRS) recording have been developed. Through fiber optics, the optogenetic stimulation was introduced into ChR2-positive glutamatergic neuron of primary motor cortex (M1). Neural and hemodynamic responses were evaluated using local field potentials (LFPs) and NIRS recordings. An in-line optical filter was applied to filter off the blue light collected from M1 and to avoid interfering the NIRS measurement. The results showed the classic waveform of evoked hemodynamic response was obtained alongside of LFPs during optogenetic stimulation. Overall, our innovative optogenetic-NIRS interface could provide long-term observation on cerebral hemodynamics during optogenetic modulation.

原文English
頁(從 - 到)83-86
頁數4
期刊IFMBE Proceedings
68
發行號3
DOIs
出版狀態Published - 2019 一月 1
事件World Congress on Medical Physics and Biomedical Engineering, WC 2018 - Prague, Czech Republic
持續時間: 2018 六月 32018 六月 8

指紋

Hemodynamics
Near infrared spectroscopy
Monitoring
Neurons
Opsins
Wave filters
Optical filters
Fiber optics
Modulation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

引用此文

@article{758afa1263b3407098add91ccd086c24,
title = "Chronically monitoring of optogenetic stimulation-induced neural and hemodynamic response",
abstract = "Understanding the link between neuronal activity and cerebral hemodynamics, known as neurovascular coupling (NVC), is critical for studying cerebral vascular dysfunctions. Optogenetics, which involves using light to control electrical activity of opsin-expressing neurons, is an approach to monitor and modulate functions of specific neurons in neuronal networks. Recent studies suggested that optogenetic stimulation induced hemodynamic response could be a good model for studying the mechanism of NVC. The aim of the study is to develop an optogenetic platform for long-term monitoring of the cerebral hemodynamics. An implantable optrode for optical stimulation and neural recording in addition to near-infrared spectroscopy (NIRS) recording have been developed. Through fiber optics, the optogenetic stimulation was introduced into ChR2-positive glutamatergic neuron of primary motor cortex (M1). Neural and hemodynamic responses were evaluated using local field potentials (LFPs) and NIRS recordings. An in-line optical filter was applied to filter off the blue light collected from M1 and to avoid interfering the NIRS measurement. The results showed the classic waveform of evoked hemodynamic response was obtained alongside of LFPs during optogenetic stimulation. Overall, our innovative optogenetic-NIRS interface could provide long-term observation on cerebral hemodynamics during optogenetic modulation.",
author = "Wu, {Chun Wei} and Jia-Jin Chen",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-981-10-9023-3_15",
language = "English",
volume = "68",
pages = "83--86",
journal = "IFMBE Proceedings",
issn = "1680-0737",
publisher = "Springer Verlag",
number = "3",

}

Chronically monitoring of optogenetic stimulation-induced neural and hemodynamic response. / Wu, Chun Wei; Chen, Jia-Jin.

於: IFMBE Proceedings, 卷 68, 編號 3, 01.01.2019, p. 83-86.

研究成果: Conference article

TY - JOUR

T1 - Chronically monitoring of optogenetic stimulation-induced neural and hemodynamic response

AU - Wu, Chun Wei

AU - Chen, Jia-Jin

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Understanding the link between neuronal activity and cerebral hemodynamics, known as neurovascular coupling (NVC), is critical for studying cerebral vascular dysfunctions. Optogenetics, which involves using light to control electrical activity of opsin-expressing neurons, is an approach to monitor and modulate functions of specific neurons in neuronal networks. Recent studies suggested that optogenetic stimulation induced hemodynamic response could be a good model for studying the mechanism of NVC. The aim of the study is to develop an optogenetic platform for long-term monitoring of the cerebral hemodynamics. An implantable optrode for optical stimulation and neural recording in addition to near-infrared spectroscopy (NIRS) recording have been developed. Through fiber optics, the optogenetic stimulation was introduced into ChR2-positive glutamatergic neuron of primary motor cortex (M1). Neural and hemodynamic responses were evaluated using local field potentials (LFPs) and NIRS recordings. An in-line optical filter was applied to filter off the blue light collected from M1 and to avoid interfering the NIRS measurement. The results showed the classic waveform of evoked hemodynamic response was obtained alongside of LFPs during optogenetic stimulation. Overall, our innovative optogenetic-NIRS interface could provide long-term observation on cerebral hemodynamics during optogenetic modulation.

AB - Understanding the link between neuronal activity and cerebral hemodynamics, known as neurovascular coupling (NVC), is critical for studying cerebral vascular dysfunctions. Optogenetics, which involves using light to control electrical activity of opsin-expressing neurons, is an approach to monitor and modulate functions of specific neurons in neuronal networks. Recent studies suggested that optogenetic stimulation induced hemodynamic response could be a good model for studying the mechanism of NVC. The aim of the study is to develop an optogenetic platform for long-term monitoring of the cerebral hemodynamics. An implantable optrode for optical stimulation and neural recording in addition to near-infrared spectroscopy (NIRS) recording have been developed. Through fiber optics, the optogenetic stimulation was introduced into ChR2-positive glutamatergic neuron of primary motor cortex (M1). Neural and hemodynamic responses were evaluated using local field potentials (LFPs) and NIRS recordings. An in-line optical filter was applied to filter off the blue light collected from M1 and to avoid interfering the NIRS measurement. The results showed the classic waveform of evoked hemodynamic response was obtained alongside of LFPs during optogenetic stimulation. Overall, our innovative optogenetic-NIRS interface could provide long-term observation on cerebral hemodynamics during optogenetic modulation.

UR - http://www.scopus.com/inward/record.url?scp=85048287997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048287997&partnerID=8YFLogxK

U2 - 10.1007/978-981-10-9023-3_15

DO - 10.1007/978-981-10-9023-3_15

M3 - Conference article

AN - SCOPUS:85048287997

VL - 68

SP - 83

EP - 86

JO - IFMBE Proceedings

JF - IFMBE Proceedings

SN - 1680-0737

IS - 3

ER -